An Overview of Aseptic Strategies Applied for In-house Polymerase Chain Reaction Techniques Discussed with Trouble Shootings

##plugins.themes.bootstrap3.article.main##

  •   Kathiravan Vayanaperumal

  •   Ranjith Kumar

Abstract

Polymerase chain reaction which is the widely utilized method had made progressive imprint in scientific research has its solid focuses and inadequacies. It presents the chance of defilement through the spreading of amplicon mist concentrates in the laboratory environment. To help lessen the probability of tainting, good laboratory practice ought to be practiced consistently. This review is centers to setting up a PCR laboratory and techniques for counteraction and devastation of contamination, and quality control.


Keywords: Polymerase Chain Reaction, PCR Lab, Aseptic Technique, Quality Control

References

Kwok S, Higuchi R. Avoiding false positives with PCR. Nature. 1989;339(6221):237. doi:10.1038/339237a0.

Aslanzadeh J. Preventing PCR amplification carryover contamination in a clinical laboratory. Ann Clin Lab Sci. 2004;34(4):389-396.

Varanat M, Maggi RG, Linder KE, Horton S, Breitschwerdt EB. Cross-contamination in the molecular detection of Bartonella from paraffin-embedded tissues. Vet Pathol. 2009;46(5):940-944. doi:10.1354/vp.08-VP-0259-B-BC.

Kalle E, Kubista M, Rensing C. Multi-template polymerase chain reaction. Biomol Detect Quantif. 2014;2: 11-29. doi:10.1016/j.bdq.2014.11.002.

Lorenz TC. Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies. J Vis Exp JoVE. 2012;(63). doi:10.3791/3998.

Guidelines for Environmental Infection Control in Health-Care Facilities: (545922006-001). Published online 2003. doi:10.1037/e545922006-001.

Bio-Pharmaceutical Cleanroom Design Guidelines. Accessed June 10, 2019. https://www.portafab.com/bio-pharmaceutical-cleanroom-design.html.

Mifflin T. Setting up a PCR laboratory. CSH Protoc. 2007;2007:pdb.top14. doi:10.1101/pdb.top14.

Fabre A-L, Colotte M, Luis A, Tuffet S, Bonnet J. An efficient method for long-term room temperature storage of RNA. Eur J Hum Genet. 2014;22(3):379-385. doi:10.1038/ejhg.2013.145.

January 7 P, Share 2014. Free Up Your Freezer with These Room-Temperature DNA Storage Solutions. Accessed June 11, 2019. http://www.biocompare.com/Editorial-Articles/152844-Free-Up-Your-Freezer-with-These-Room-Temperature-DNA-Storage-Solutions/.

7 Tips to Make PCR Primers Last Longer and PCR Reactions Run Better. GEN - Genetic Engineering and Biotechnology News. Published June 27, 2012. Accessed June 11, 2019. https://www.genengnews.com/magazine/7-tips-to-make-pcr-primers-last-longer-and-pcr-reactions-run-better/.

PCR Troubleshooting | LSR | Bio-Rad. Accessed June 11, 2019. http://www.bio-rad.com/en-us/applications-technologies/pcr-troubleshooting?ID=LUSO3HC4S.

Pushparaj PN. Revisiting the Micropipetting Techniques in Biomedical Sciences: A Fundamental Prerequisite in Good Laboratory Practice. Bioinformation. 2020;16(1):8-12. doi:10.6026/97320630016008.

Standard_Operating_Procedures.pdf. Accessed June 11, 2019. https://www.icmr.nic.in/sites/default/files/guidelines/Standard_Operating_Procedures.pdf.

##plugins.themes.bootstrap3.article.details##

How to Cite
Vayanaperumal, K., & Kumar, R. (2020). An Overview of Aseptic Strategies Applied for In-house Polymerase Chain Reaction Techniques Discussed with Trouble Shootings. European Journal of Biology and Biotechnology, 1(6). https://doi.org/10.24018/ejbio.2020.1.6.119