Research Article

Effects of Cytokinin on in vitro Propagation of Bauhinia variegata L.

Belai Meeta Suwal Singh
Patan Multiple Campus, Nepal
* Corresponding author
DOI icon 10.24018/ejbio.2020.1.6.123

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Submitted 2020-11-11
Published 2020-11-28

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Article Main Content

Mature seeds of Bauhinia variegata L were cultured on half strength Murashige and Skoog medium. For experimentation, nodal cuttings were used as explants from in vitro growing plants. Cytokinin, N-benzyl-9-(2-tetrahydropyranyl) (BPA), kinetin(6-furfurylaminopurine), zeatin, 6-(4-hydroxy-3-methyl-trans -2-butenyl amino purine), 2- isopentenyl amino purine (2-ip), and benzylaminopurine (BAP) were tested for best propagation. Well grown plants were achieved in medium supplemented with 5 µM BPA and 0.5 µM BAP. The propagated plants were acclimatized very well after transferred to the field.

Keywords: MS medium, nodal culture, BPA, Kinetin, Zeatin, 2-ip and BAP

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