Characterization of Antimicrobial Peptides from Local Forest Dwelling Ants: In-vitro Screening for Antimicrobial Activity

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  •   Barna Chakraborty

  •   Debashis Gayen

  •   C. Deeya Ghosh Dastidar

Abstract

Antimicrobial peptides (AMPs) recognized as host defense peptide (HDPs), have achieved considerable attention during last two decades as potential components of the instinctive immune system. Amongst the insects over casted for AMPS the hymenopterans comprising of ants have been identified for possessing potent antimicrobial peptides, those are small linear peptides. An investigation was taken up to screen for antimicrobial peptides collected from ants thriving in Forest Plantation in Hijli of Midnapore District, W.B. Three species of ants, members of the family Formicidae, were collected for in vitro antimicrobial activities of the whole body extract. These were tested by in the 10% of acetic acid solution using the Kirby-Bauer disc diffusion method against test microorganism viz. Escherichia coli, Staphylococcus mutans, Vibrio cholarae and Candida albicans. A strong antimicrobial activity was noted in all the extracts against all the microorganisms. The Spectrophotometric scanning was carried out to confirm the presence of peptides. TLC and HPLC were done. The result revealed that all the three species of ants exploited in this study were found to be a promising source of antimicrobial peptides.


Keywords: Antimicrobial peptides, Escherichia coli, Formicidae, HPLC, TLC

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How to Cite
Chakraborty, B., Gayen, D., & Dastidar, C. D. G. (2021). Characterization of Antimicrobial Peptides from Local Forest Dwelling Ants: In-vitro Screening for Antimicrobial Activity. European Journal of Biology and Biotechnology, 2(1), 20-25. https://doi.org/10.24018/ejbio.2021.2.1.138