Characterization of Olive (Olea Europaea L.) Genetic Resources via PCR-Based Molecular Marker Systems


  •   S. Galatali

  •   N. Abdul Ghafoor

  •   E. Kaya


Olea europaea L., which is one of the ancient culture species cultivated in the Mediterranean area, has approximately 1200 cultivars. Its wild thype forms from O. europaea subsp. europaea var. sylvestris and its culture type forms from O. europaea subsp. europaea var. europaea. Olive cultivation is multiplied by grafting or cutting whereas wild type olive seedlings derived from seeds of O. europaea L. var. sylvestris. Because they have very big level heterozygosities, the genetic diversity of olive cultivars is quite high that they are predominantly allogamus. This genetic variability causes many confusions for identification of olive cultivars and since both oil quality and olive productivity are traits inherited to a variety, it is urgently needed to solve characterization and evaluation of olive genetic resources. The molecular marker systems are independent from environmental factors and effective technology to both detect homonymous and synonymous of cultivars and identify olive varieties. The current review is aimed to present molecular marker systems for identification and characterization of olive cultivars and emphasize their application for conservation of olive germplasm.

Keywords: Molecular Characterization, Olive (Olea europaea L.), PCR-Based Marker System


G. Bartolini and R. Petruccelli, Classification, Origin, Diffusion, and History of the Olive, Rome, Food and Agriculture Organization of the United Nations, 2002, pp. 43.

D. Zohary and P. Spiegel-Roy. “Beginning of Fruit Growing in the Old World,” Science, vol.187, pp. 319-327, 1975.

G. Bartolini, G. Prevost, C. Messeri and G. Carignani. Olive Germplasm: Cultivars and World-Wide Collections. FAO, Rome, 1998.

D. Barranco, A. Cimato, P. Fiorino, L. Rallo, A. Touzani, C. Castaneda and I. Trujillo. “World Catalogue of Olive Varieties,” International Olive Oil Council, Madrid, Espanha. 2000.

M.G. Claros, R. Crespillo, M.L. Aguilar and F.M. Cánovas. “DNA Fingerprinting and Classification of Geographically Related Genotypes of Olive-Tree (Olea europaea L.),” Euphytica, vol. 116, pp. 131-142, 2000.

D. Barranco and L. Rallo. “Las Variedades de Olivo Cultivadas en Andalucia Consejeria de la Junta de Andalucia,” Madrid. MAPA, 1984.

G. Prevost, G. Bartolini and C. Messeri. “Cultivar Italiane di Olivo e Loro Sinonimi,” Lucca. Menegazzo, 1993.

T. Ganino, G. Bartolini and A. Fabbri. “The Classification of Olive Germplasma Review,” The Journal of Horticultural Science and Biotechnology, vol. 81, no. 3. pp. 319-334, 2006.

G. Caruso. “Monografia dell’ Olivo,” In Enciclopedia agnia Italiano. 3(5), UTET, Turin, Italy. 1983.

C.A. Pontikis, M. Loukas and G. Kousounis. “The Use of Biochemical Markers to Distinguish Olive Cultivars,” The Journal of Horticultural Science, vol. 55, no. 4. pp. 333-343, 1980.

I. Trujillo, L. Rallo, E.A. Carbonell and M.J. Asins. “Isoenzymatic Variability of Olive Cultivars According to Their Origin,” Acta Horticulturae, vol. 286, pp. 137-140, 1990.

N. Ouazzani, R. Lumaret, P. Villemur and F. Di Giusto. “Leaf Allozyme Variation in Cultivated and Wild Olive Trees (Olea europaea L.),” Journal of Heredity, vol. 84, pp. 34-42, 1993.

M. Durante, E. Attina, S. Nardi, G. Cacco. “Changes Induced by Humic Substances and Nitrate in the Genomic Structure of Wheat Roots,” In: Senesi, N., Miano, T.M., (Eds.), Sixth International Meeting of IHSS, Monopoli (Bari), pp. 149. 1992.

W. Povel, M. Morgante, C. Andre, M. Hanafey, J. Vogel, S. Tingay and A. Rafalski. “The Comparisons of RFLP, RAPD, AFLP, and SSR (Microsatellite) Markers for Germplasm Analysis,” Molecular Breeding, vol. 2, pp. 225-238, 1996.

G. Besnard and A. Berville. “On Chloroplast DNA Variations in the Olea europaea L. Complex: Comparison of RFLP and PCR Polymorphisms,” Theoretical and Applied Genetics, vol. 104, pp. 1157-1163, 2002.

G. Besnard, C. Breton, P. Baradat, B. Khadari and A. Bervillé. “Cultivar Identification in Olive Based on RAPD Marker,” Journal of the American Society for Horticultural Science, vol. 126, no. 6. pp. 668-675, 2001.

P. Hatzopoulos, G. Banilas, K. Giannoulia, F. Gazis, N. Nikoloudakis, D. Milioni and K. Haralampidis. “Breeding, Molecular Markers and Molecular Biology of the Olive Tree,” European Journal of Lipid Science and Technology, vol. 104, pp. 574-586, 2002.

R. Testolin, M.T. Marrazzo, G. Cipriani, R. Quarta, I. Verde, M.T. Dettori, M. Pancaldi and S. Sansavini. “Microsatellite DNA in Peach (Prunus persica L. Batsch) and its Use in Fingerprinting and Testing the Genetic Origin of Cultivars,” Genome, vol. 43, pp. 512-520, 2000.

M. Gallitelli, L. Semeraro and N.M. Antonelli. RFLP Analysis in the Olive (Olea europaea L.), EMBO Course, 1991, Cologne.

J.G.K. Williams, A.R. Kubelik, K.J. Livak, J.A. Rafalski, S.V. Tingey. “DNA Polymorphisms Amplified by Arbitrary Primers are Useful as Genetic Markers,” Nucleic Acids Research, vol. 18, pp. 6531-6535, 1990.

J. Welsh and M. McClelland. “Fingerprinting Genomes Using PCR with Arbitrary Primers,” Nucleic Acids Research, vol. 18, pp. 7213-7218, 1990.

P. Bogani, D. Cavalieri, R. Petruccelli, L. Polsinelli and G. Roselli. “Identification of Olive Tree Cultivars by Using Random Amplified Polymorphic DNA,” Acta Horticulturae, vol. 356, pp. 98-101, 1994.

A. Fabbri, J.I. Hormaza and V.S. Polito. “Random Amplified Polymorphic DNA Analysis of Olive (Olea europaea L.) Cultivars,” Journal of the American Society for Horticultural Science, vol. 120, pp. 538-542, 1995.

E. Perri, R. Sirianni, G. Godino and S. Tartarini. “Characterization of Italian Olive (Olea europaea L.) Cultivars by Satistical and Molecular Methods,” Acta Horticulturae, vol. 474, no. 2. pp. 489-493, 1999.

M. Gonzalo-Claros, Crespillo, R. Aguilar, M.L. Cánovas and F.M. “DNA Fİngerprinting and Classification of Geographically Related Genotypes of Olive-Tree (Olea europaea L.),” Euphytica, vol. 116, pp. 131-142, 2000.

A. Belaj, I. Trujillo, R. De la Rosa, L. Rallo and M.J. Gime´nez. “Polymorphism and Discrimination Capacity of Randomly Amplified Polymorphic Markers in an Olive Germplasm Bank,” Journal of the American Society for Horticultural Science, vol. 126, pp. 64-71, 2001.

R.J. Mailer and C.E. May. “Variability and Interrelationships of Olive Trees and Cultivars Using RAPD Analysis,” Advances in Horticultural Sciences, vol. 16, no. 3-4. pp. 192-197, 2002.

A. Fabbri and T. Ganino, “Il Germoplasma Olivicolo in Emilia,” Atti Convegno Germoplasma Olivicolo e Tipicita` dell’olio Perugia, 2003, pp 48-52.

N. Mir Ali and I. Nabulsi. “Genetic Diversity of Almonds (Prunus dulcis) Using RAPD Technique,” Scientia Horticulturae, vol. 98, pp. 461-471, 2003.

T. Ganino and A. Fabbri, “Genetic Characterization of Olea europaea L. Germplasm in Northern Italy,” 5th International Symposium on Olive Growing, Izmir (Turkey), 27 September-2 October, 2004.

V.J. Gemas, M.J. Rijo-Johansen, R. Tenreiro and P. Fevereiro. “Inter-Varietal and Intra-Varietal Analysis of 3 Olea europaea L. Cultivars Using the RAPD Technique,” The Journal of Horticultural Science and Biotechnology, vol. 75, pp. 312-319, 2000.

G. Roselli, L. Petruccelli, L. Polsinelli and D. Cavalieri. “Variability in Five Tuscan Olive Cultivars (Olea europaea L.),” Journal of Genetics and Breeding, vol. 56, pp. 51-60, 2002.

G. Besnard, B. Khadari, P. Baradat and A. Berville´. “Olea europaea (Oleaceae) Phylogeography Based on Chloroplast DNA Polymorphism,” Theoretical and Applied Genetics, vol. 104, pp. 1353-1361, 2002.

D.G. Ranamukhaarachchi, M.E. Kane, C.L. Guy and Q.B. Li. “Modified AFLP Technique for Rapid Genetic Characterization in Plants,” BioTechniques, vol. 29, pp. 858-866, 2000.

P. Vos, R. Hogers, M. Bleeker, M. Reijans, T. Vande, Lee, M.Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper and M. Zabeau. “AFLP: a New Technique for DNA Fingerprinting,” Nucleic Acids Research, vol. 23, pp. 4407-4414, 1995.

R.R. Prabhu and P.M. Gresshoff. “Inheritance of Polymorphic Markers Generated by DNA Amplification Fingerprinting and Their Use as Genetic Markers in Soybean,” Plant Molecular Biology, vol. 26, pp. 105-116, 1994.

J. Lu, M.R. Knox, M.J. Ambrose, J.K.M. Brown and T.H.N. Ellis. “Comparative Analysis of Genetic Diversity in Pea Assessed by RFLP-and PCR-Based Methods,” Theoretical and Applied Genetics, vol. 93, pp. 1103-1111, 1996.

M. Hill, H. Witsenboer, M. Zabeau, P. Vos, R. Kesseli and R. Michelmore. “PCR-Based Fingerprinting Using AFLPs as a Tool for Studying Genetic Relationships in Lactuca spp.,” Theoretical and Applied Genetics, vol. 93, pp. 1202-1210, 1996.

S.K. Sharma, M.R. Knox and T.H.N. Ellis. “AFLP Analysis of the Diversity and Phylogeny of Lens and its Comparison with RAPD Analysis,” Theoretical and Applied Genetics, vol. 93, pp. 751-758, 1996.

D. Milbourne, R. Meyer, J.E. Bradshaw, E. Baird, N. Bonar, J. Provan, W. Powell and R. Waugh. “Comparison of PCR-Based Marker Systems for the Analysis of Genetic Relationships in Cultivated Potato,” Molecular Breeding, vol. 3, pp. 127-136, 1997.

A. Angiolillo, M. Mencuccini and L. Baldoni. “Olive Genetic Diversity Assessed Using Amplified Fragment Length Polymorphisms,” Theoretical and Applied Genetics, vol. 98, pp. 411-421, 1999.

O. Ambrosino and R. Rao, “Marcatori AFLP per la Valutazione Della Variabilità Genetica Intra-Intervarietale in Cultivar di Olivo,” Atti VI Convegno Nazionale Biodiversità, 2001, vol 2, pp. 397-405.

P. Resta, C. Lotti, G. Fanizza, A. Godini, R. Mariani and M. Palasciano. “Use of AFLP to Characterize Apulian Olive Varieties,” Acta Horticulturae, vol. 586, pp. 73-77, 2002.

P.J. Maughan, M.A. Saghai, G.R. Maroof and G.M. Buss, Huestis. “Amplified Fragment Length Polymorphism (AFLP) in Soybean: Species Diversity, Inheritance and Near-Isogenic Line Analysis,” Theoretical and Applied Genetics, vol. 93, pp. 392-401. 1996.

V. Hongtrakul, G.M. Huestis, S. Knapp. “Amplified Fragment Length Polymorphism as a Tool for DNA Fingerprinting Sun-Flower Germplasm: Genetic Diversity among Oilseed Inbred Lines,” Theoretical and Applied Genetics, vol. 95, pp. 400-407, 1997.

M.J. Aranzana, A. Pineda, P. Cosson, E. Dirlewanger, J. Ascasibar, G. Cipriani, C.D. Ryder, R. Testolin, A. Abbott, G.J. King, A.F. Iezzoni and P. Arús, A “Set of Simple-Sequence Repeat (SSR) Markers Covering the Prunus Genome,” Theoretical and Applied Genetics, vol. 106, pp. 819-825, 2003.

A.M. Cheng, M.W. Byrom, J. Shelton and L.P. Ford. “Antisense Inhibition of Human miRNAs and Indications for an Involvement of miRNA in Cell Growth and Apoptosis,” Nucleic Acids Research, vol. 33, pp. 1290-1297, 2005.

T. Thiel, W. Michalek, R.K. Varshney and A. Graner. “Exploiting EST Databases for the Development and Characterization of Gene-Derived SSR-Markers in Barley (Hordeum vulgare L.),” Theoretical and Applied Genetics, vol. 106, pp. 411-422, 2003.

M. Morgante, A. Pfeiffer, I. Jurman, G. Paglia and A.M. Olivieri, “Isolation of Microsatellite Markers in Plants,” in Molecular Tools for Screening Biodiversity, A. Karp, P.G. Isaac, D.S. Ingram, Ed. Plants and Animals, 1998, pp. 75-134.

F. Lefort, S. Brachet, N. Frascaria-Lacoste, K.J. Edwards, G.C. Douglas. “Identification and Characterization of Microsatellite Loci in Ash (Fraxinus excelsior L.) and Their Conservation in the Olive Family (Oleaceae),” Molecular Ecology, vol. 8, pp. 1075-1092, 1999.

P. Rallo, G. Dorado and A. Martin. Development of Simple Sequence Repeats (SSRs) in Olive Tree (Olea europaea L.),” Theoretical and Applied Genetics, vol. 101, pp. 984-989, 2000.

K.M. Sefc, M.S. Lopes, D. Mendonça, M. Rodrigues Dos Santos, M. Laimer Da Camara Machado and A. Da Camara Machado. “Identification of Microsatellite Loci in Olive (Olea europaea) and Their Characterization in Italian and Iberian Olive Trees,” Molecular Ecology, vol. 9, pp. 1171-1173, 2000.

G. Cipriani, M.T. Marrazzo, G. Di Gaspero and R. Testolin. “DNA Microsatellite in Fruit Crops: Isolation, Length Polymorphism, Inheritance, Somatic Stability, and Cross-Species Conservation,” Acta Horticulturae, vol. 546, pp. 145-150, 2000.

F. Carriero, G. Fontanazza, F. Cellini and G. Giorio. “Identification of Simple Sequence Repeats (SSRs) in Olive (Olea europaea L.),” Theoretical and Applied Genetics, vol. 104, pp. 301-307, 2002

R. De la Rosa, C. James and K.R. Tobutt. “Isolation and Characterization of Polymorphic Microsatellite in Olive (Olea europaea L.) and Their Transferability to Other Genera in the Oleaceae,” Primer Notes. Molecular Ecology Notes, vol. 2, pp. 265-267, 2002.

D. Bandelj, J. Jakse and B. Javornik. “DNA fingerprinting of Olive Varieties by Microsatellite Markers,” Food Technology and Biotechnology, vol. 40, no. 3. pp. 185-190, 2002.

A. Belaj, G. Cipriani, R. Testolin, L. Rallo and I. Trujillo. “Characterization and Identification of the Main Spanish and Italian Olive Cultivars by Simple-Sequence-Repeat Markers,” HortScience, vol. 39, no. 7. pp. 1557-1561, 2004.

M. La Mantia, O. Lain, T. Caruso, R. Testolin. “SSR-Based DNA Fingerprints Reveal the Genetic Diversity of Sicilian Olive (Olea europaea L.) Germplasm,” The Journal of Horticultural Science and Biotechnology, vol. 80, pp. 628-632, 2005.

E. Zietkiewicz, A. Rafalski and D. Labuda. “Genome Fingerprinting and Simple Sequence Repeat (SSR)-Anchored Polymerase Chain Reaction Amplification,” Genomics, vol. 20, pp.176-183, 1994.

P.J. Terzopoulos, B. Kolano, P.J. Bebeli, P.J. Kaltsikes and I. Metzidakis. “Identification of Olea europaea L. Cultivars Using Inter-Simple Sequence Repeat Markers,” Scientia Horticulturae, vol. 105, pp. 45-51, 2005.

V.J.V. Gemas, M.C. Almadanim, R. Tenreiro, A. Martins and P. Fevereiro. “Genetic Diversity in the Olive Tree (Olea europaea L. subsp. europaea) Cultivated in Portugal Revealed by RAPD and ISSR Markers,” Genetic Resource and Crop Evolution, vol. 5, pp. 501-511, 2004.

S. Gomes, P. Martins-Lopes, J. Lima-Brito, J. Meirinhos, J. Lopes, A. Martins and H. Guedes- Pinto. “Evidence of Clonal Variation in Olive ‘Verdeal-Transmontana’ Cultivar Using RAPD, ISSR and SSR Markers,” Journal of Horticultural Science and Biotechnology, vol. 83, no. 4. pp. 395-400, 2008.

P. Martins-Lopes, J. Lima-Brito, S. Gomes, J. Meirinhos, L. Santos and H. Guedes-Pinto. “RAPD and ISSR Molecular Markers in Olea europaea L.: Genetic Variability and Cultivar Identification,” Genetic Resource and Crop Evolution, vol. 54, pp. 117-128, 2007.

P. Martins-Lopes, S. Gomes, J. Lima-Brito, J. Lopes and H. Guedes-Pinto. “Assessment of Clonal Genetic Variability in Olea europaea L. ‘Cobrançosa’ by Molecular Markers,” Scientia Horticulturae, vol. 123, pp. 82-89, 2009.

E. Kaya. “ISSR Analysis for Determination of Genetic Diversity and Relationship in Some Turkish Olive (Olea europaea L) Cultivars,” Notulae Botanicae Horti Agrobotanici Cluj-Napoca, vol. 43, no. 1. pp. 96-99, 2015.

J. Hess, J.W. Kadereit and P. Vargas. “The Colonization History of Olea europaea L. in Macaronesia Based on Internal Transcribed Spacer 1 (ITS-1) Sequences, Randomly Amplified Polymorphis DNAs (RAPD), and Intersimple Sequence Repeats (ISSR),” Molecular Ecology, vol. 9, pp. 857-868, 2000.

A. Pasqualone, F. Caponio and A. Blanco. “Inter-Simple Sequence Repeat DNA Markers for Identification of Drupes from Different Olea europaea L. Cultivars,” European Food Research Technology, vol. 213, pp. 240-243, 2001.

S. Pafundo, C. Agrimonti, E. Maestri, N. Marmiroli. “Applicability of SCAR Markers to Food Genomics: Olive Oil Traceability,” Journal of Agricultural and Food Chemistry, vol. 5, pp. 6052-6059, 2007.

M. Marieschi, A. Torelli, A. Bianch and R. Bruni. “Development of a SCAR Marker for the Identification of Olea europaea L.: A Newly Detected Adulterant in Commercial Mediterranean Oregano,” Food Chemistry, vol. 126, no. 2. pp. 705-709, 2011.

P. Hernández, A. Martín and G. Dorado. “Development of SCARs by Direct Sequencing of RAPD Products: Practical Tool for the Introgression and Marker-Assisted Selection of Wheat,” Molecular Breeding, vol. 5, pp. 245-253, 1999.

J.R. Vidal, P. Delavault, M. Coarer and A. Defontaine. “Design of Grapevine (Vitis vinifera L.) Cultivar-Specific SCAR Primers for PCR Fingerprinting,” Theoretical and Applied Genetics, vol. 101, pp. 1194-1201, 2000.

Y. Zhang and J.R. Stommel. “Development of SCAR and CAPS Markers Linked to the Beta Gene in Tomato,” Crop Science, vol. 41, pp. 1602-1608, 2001.

G.P. Lee, C.H. Lee and C.S. Kim. “Molecular Markers Derived from RAPD, SCAR, and the Conserved 18S rDNA Sequences for Classification and Identification in Pyrus pyrifolia and P. Communis,” Theoretical and Applied Genetics, vol. 108, pp. 1487-1491, 2004.

R. Bautista, R. Crespillo and F.M. Canovas. “Identification of Olive-Tree Cultivars with SCAR Markers,” Euphytica, vol. 129, pp. 33-41, 2003.

M. Busconi, L. Sebastiani and C. Fogher. “Development of SCAR Markers for Germplasm Characterisation in Olive Tree (Olea europaea L.),” Molecular Breeding, vol. 17, no. 1. pp. 59-68, 2006.

P. Hernández, R. de la Rosa, L. Rallo, and G. Dorado. “Development of SCAR Markers in Olive (Olea europaea) by Direct Sequencing of RAPD Products: Applications in Olive Germplasm Evaluation and Mapping,” Theoretical and Applied Genetics, vol. 103, pp. 788-791, 2001.

M. Agarwal, N. Shrivastava and H. Padh. “Advances in Molecular Marker Techniques and Their Applications in Plant Sciences,” Plant Cell Reports, vol. 27, pp. 617-631, 2008.

S. Reale, S. Doveri, A. Diaz, A. Angiolillo, L. Lucentini, F. Pilla, A. Martín, P. Donini and D. Lee. “SNP-Based Markers for Discriminating Olive (Olea europaea L.) Cultivars,” Genome, vol. 49, no. 9. pp. 1193-1205, 2006.

A. Katsiotis, T. Schmidt and J.S. Heslop-Harrison. “Chromosomal and Genomic Organization of Ty1-Copia-Like Retrotransposon Sequences in the Genus Avena,” Genome, vol. 39, pp. 410-417, 1996.

A. Suoniemi, K. Anamthawat-Jonsson, T. Arna and A.H. Schulman. “Retrotransposon BARE-1 is a Major, Dispersed Component of the Barley (Hordeum vulgare L.) genome,” Plant Molecular Biology, vol. 30, pp. 1321-1329, 1996.

A.J. Flavell, E. Dunba, R. Anderson, S.R. Pearce, R. Hartley and A. Kumar. “Ty1-Copia Group Retrotransposons are Ubiquitous and Heterogeneous in Higher Plants,” Nucleic Acids Research, vol. 20, pp. 3639-3644, 1992.

D.F. Voytas, M.P. Cummings, A.K. Konieczny, F.M. Ausubel and S.R. Rodermel. “Copia-Like Retrotransposons are Ubiquitous Among Plants,” Proceedings of the National Academy of Sciences, vol. 89, pp. 7124-7128, 1992.

S.R. Pearce, U. Pich, G. Harrison, A.J. Flavell, J.S. Heslop-Harrison, I. Schubert and A. Kumar. “The Ty1-Copia Group Retrotransposons of Allium cepa are Distributed Troughout the Chromosomes but are Enriched in the Terminal Heterochromatin,” Chromosome Research, vol. 4, pp. 357-364, 1996.

S.R. Pearce, G. Harrison, J.S. Heslop-Harrison, A.J. Flavell and A. Kumar. “Characterisation and Genomic Organisation of Ty1-Copia Group Retrotransposons in Rye (Secale cereale),” Genome, vol. 40, pp. 1-9, 1997.

S.E. Adams, J. Mellor, K. Gull, R.B. Sim, M.F. Tuite, S.M. Kingsman and A.J. Kingsman. “The Functions and Relationships of Ty-VLP Proteins in Yeast reflect Those of Mammalian Retroviral Proteins,” Cell, vol. 49, pp. 111-119, 1987.

M. Shimamura, H. Yasue, K. Ohshima, H. Abe, H. Kato, T. Kishiro, M. Goto, I. Munechika and N. Okada. “Molecular Evidence from Retroposons that Whales form a Clade within Even-Toed Ungulates,” Nature, vol. 388, pp. 666-670, 1997.

B. Koksal, E. Kaya, K. Efe, B. Uzan and Y. Ozden-Ciftci. “Molecular Characterization of Some Turkish Olive (Olea europaea L.) Cultivars Based on IRAP Markers,” 2014 World Forum on Biology, Joint Meeting of the Society for In Vitro Biology and the Society for Cryobiology, Savannah, Georgia, USA, May 31-June 4, 2014.

E. Kaya and E. Yılmaz Gökdoğan. “Using Two Retrotransposon Based Marker Systems (IRAP and REMAP) for Molecular Characterization of Olive (Olea europaea L.) Cultivars,” Notulae Botanicae Horti Agrobotanici Cluj-Napoca, vol. 44, no. 1. pp. 167-174, 2016.

M.T. Ozkaya, E. Cakir, Z. Gokbayrak, H. Ercan and N. Taskin. “Morphological and Molecular Characterization of Derik Halhali Olive (Olea europaea L.) Accessions Grown in Derik–Mardin Province of Turkey,” Scientia Horticulturae, vol. 108, pp. 205-209, 2006.

N. Grati-Kamoun, F. Lamy Mahmoud, A. Rebaı, A. Gargouri, O. Panaud and A. Saar. “Genetic Diversity of Tunisian Olive Tree (Olea europaea L.) Cultivars Assessed by AFLP Markers,” Genetic Resources and Crop Evolution, vol. 53, pp. 265-275, 2006.

A. Belaj, C. Munoz-Diez, L. Baldoni, A. Porceddu, D. Barranco and Z. Satovic. “Genetic Diversity and Population Structure of Wild Olives from the North-Western Mediterranean Assessed by SSR Markers,” Annals of Botany, vol. 100, pp. 449-458, 2007.

S. Stambuk, D. Sutlović, P. Bakarić, S. Petričević and Š. Anđelinović. “Forensic Botany: Potential Usefulness of Microsatellite-Based Genotyping of Croatian Olive (Olea europaea L.) in Forensic Casework,” Croatian Medical Journal, vol. 48, pp. 556-562, 2007.

L. Natali, T. Giordani, M. Buti and A. Cavallini. “Isolation of Ty1-Copiaputative LTR Sequences and Their Use as a Tool to Analyse Genetic Diversity in Olea europaea,” Molecular Breeding, vol. 19, pp. 255-265, 2007.

C. Breton, C. Pinatel, F. Medail, F. Bonhomme and A. Berville. “Comparison Between Classical and Bayesian methods to Investigate the History of Olive Cultivars Using SSR-Polymorphisms,” Plant Science, vol. 175, pp. 524-532, 2008.

S. Doveri, F.S. Gil, A. Dıaz, S. Reale, M. Busconi, A.C. Machado, A. Martın, C. Fogher, P. Donini and D. Lee. “Standardization of a Set of Microsatellite Markers for Use in Cultivar Identification Studies in Olive (Olea europaea L.),” Scientia Horticulturae, vol. 116, pp. 367-373, 2008.

D. Poljuha, B. Sladonja, E. Setic, A. Milotic, D. Bandelj, J. Jakse and B. Javornik. “DNA Fingerprinting of Olive Varieties in Istria (Croatia) by Microsatellite Markers,” Scientia Horticulturae, vol. 115, pp. 223-230, 2008.

H. Hannachi, C. Breton, M. Msallem, S. Ben El Hadj, M. El Gazzah and A. Berville. “Differences Between Native and Introduced Olive Cultivars as Revealed by Morphology of Drupes, Oil Composition and SSR Polymorphisms: A Case Study in Tunisia,” Scientia Horticulturae, vol. 116, pp. 280-290, 2008.

B. Zitoun, V. Bronzini de Caraffa, J. Giannettini, C. Breton, A. Trigui, J. Maury, C. Gambotti, B. Marzouk and L. Berti. “Genetic Diversity in Tunisian Olive Accessions and Their Relatedness with Other Mediterranean Olive Genotypes,” Scientia Horticulturae, vol. 115, pp. 416-419, 2008.

T. Bracci, L. Sebastiani, M. Busconi, C. Fogher, A. Belaj and I.Trujillo. “SSR Markers Reveal the Uniqueness of Olive Cultivars from the Italian Region of Liguria,” Scientia Horticulturae, vol.122, pp. 209-215, 2009.

V. Alba, C. Montemurro, W. Sabetta, A. Pasqualone and A. Blanco. “SSR-based Identification Key of Cultivars of Olea europaea L. Diffused in Southern-Italy,” Scientia Horticulturae, vol. 123, pp. 11-16, 2009.

S. Gomes, P. Martins-Lopes, J. Lopes and H. Guedes-Pinto. “Assessing Genetic Diversity in Olea europaea L. Using ISSR and SSR Markers,” Molecular Biology Reports, vol. 27, pp. 365-373, 2009.

I. Muzzalupo, F. Stefanizzi and E. Perri. “Evaluation of Olives Cultivated in Southern Italy by Simple Sequence Repeat Markers,” Hortscience, vol. 44, no. 3. pp. 582-588, 2009.

M. Sesli and E.D. Yeğenoğlu. “RAPD Assay of Wild-Type Olives in Turkey,” Genetics and Molecular Research, vol. 9, no. 2. pp. 966-972, 2010.

A. Belaj, C. Munoz-Diez, L. Baldoni, Z. Satovic and D. Barranco. Genetic “Diversity and Relationships of Wild and Cultivated Olives at Regional Level in Spain,” Scientia Horticulturae, vol. 124, pp. 323-330, 2010.

C. Durgac, Y. Kiyga and M. Ulas. “Comparative Molecular Analysis of Old Olive (Olea europaea L.) Genotypes from Eastern Mediterranean Region of Turkey,” African Journal of Biotechnology, vol. 9, no. 4. pp. 428-433, 2010.

M. Sesli and E.D. Yeğenoğlu. “Comparison of Manzanilla and Wild Type Olives by RAPD-PCR Analysis,” African Journal of Biotechnology, vol. 9, no. 7. pp. 986-990, 2010.

E. Albertini, R. Torricelli, E. Bitocchi, L. Raggi, G. Marconi, L. Pollastri, G. Di Minco, A. Battistini, R. Papa and F. Veronesi. “Structure of Genetic Diversity in Olea europaea L. Cultivars from Central Italy,” Molecular Breeding, vol. 27, pp. 533-547, 2011.

G. Corrado, A. Imperato, M. La Mura, E. Perri and R. Rao. “Genetic Diversity Among Olive Varieties of Southern Italy and the Traceability of Olive Oil Using SSR Markers,” Journal of Horticultural Science & Biotechnology, vol. 86, no. 5. pp. 461-466, 2011.

S. Ercisli, A. Ipek and E. Barut. “SSR Marker-Based DNA Fingerprinting and Cultivar Identification of Olives (Olea europaea),” Biochemical Genetics, vol. 49, pp. 555-561, 2011.

A.A. Awan, M. Zubair, A. Iqbal, S.J. Abbas and N. Ali. “Molecular Analysis of Genetic Diversity in Olive Cultivars,” African Journal of Agricultural Research, vol. 6, no. 21. pp. 4937-4940, 2011.

A.H. Beiki, S. Saboor and M. Ebrahimi. “A New Avenue for Classification and Prediction of Olive Cultivars Using Supervised and Unsupervised Algorithms,” Plos One, vol. 7, no.9. pp. 1-9, 2012.

S.G. Atienza, R. De la Rosa, M.C. Domínguez-García, A. Martín, A. Kilian and A. Belaj. “Use of DArT Markers as a Means of Better Management of the Diversity of Olive Cultivars,” Food Research International, vol. 54, pp. 2045-2043, 2013.

E. Figueiredo, J. Canhoto and M.M. Ribeiro. “Fingerprinting and Genetic Diversity of Olea europaea L. ssp. europaea Accessions from the Cultivar Galega Using RAPD Markers,” Scientia Horticulturae, vol. 156, pp. 24-28, 2013.

A. Cicatelli, T. Fortunati, I. De Feis and S. Castiglione. “Oil Composition and Genetic Biodiversity of Ancient and New Olive (Olea europea L.) Varieties and Accessions of Southern Italy,” Plant Science, vol. 210, pp. 82-92, 2013.

A. Belaj, M.A. Ojeda, C. Muñoz, E. Rodríguez, A. Díaz, P. Rallo, R. De la Rosa, D. Barranco, L. Rallon and I. Trujillo. “The Use of Molecular Markers to Characterize Olive (Olea europaea L.) Germplasm and its Wild Relatives,” OliveBioteq, pp. 391-396, 2006.


How to Cite
Galatali, S., Abdul Ghafoor, N., & Kaya, E. (2021). Characterization of Olive (Olea Europaea L.) Genetic Resources via PCR-Based Molecular Marker Systems. European Journal of Biology and Biotechnology, 2(1), 26-33.