Assessment of Microbiological Quality and Safety during the Processing of Traditional Beers made from Sorghum in the “Mandara” Mountains of the Far-North Region of Cameroon



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Submitted Feb 17, 2021
Published Apr 8, 2021
10.24018/ejbio.2021.2.2.156

Abstract viewed = 905 times

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Indigenous beers are very popular and widely consumed by people from northern Cameroon because of its low cost. Despite their appeal, microbial quality of these beverages remains a serious call for concern. This work was aimed to investigate microbial changes and hazards of contamination during the processing of two special sorghum beers brewed in northern Cameroon. Producers were observed during the production and samples were collected at different stages for analysis of microbiological parameters such as total count, fungi, spore-forming bacteria, Coliforms and E. coli using referenced methods. Total count ranged from 13.5 x 103 to 195 x 103 CFU/mL and 0.16 x 103 to 660 x 103 CFU/mL; fungi from 0.26 x 103 to 22 x 103 CFU/mL and 0.22 x 103 to 85 x 103 CFU/mL; E. coli from 0.69 x 102 to 13.6 x 102 CFU/mL and 0.65 x 102 to 3.8 x 102 CFU/mL during the production of the red “té” and white “mepdli” beers, respectively. Spore-forming bacteria and Coliforms (total and fecal) were detected in all the collected samples. Bacterial spores were also enumerated in red (38 x 103 CFU/g) and white (62.5 x 103 CFU/g) sorghum grains used as the main raw materials for the production of “té” and “mpedli” beers. The results suggest that the processing of both turbid beers using the traditional method are exposed to microbial contamination. Increase of microbial loads after soaking, adding of the supernatant (red beer only) and malted flour (white beer only) after the heating step means that control measures are needed to prevent contamination after these sensitive stages. Proper handling of raw materials, adequate implementation of heating and fermentation were found as effective critical control points. Training of producers on the hazards analysis and good manufacturing and hygiene practices have been suggested as strategies to improve the safety of indigenous beers.

Keywords: Northern Cameroon, sorghum, traditional beers, processing, microbial contamination, critical control point, food safety

References

  1. K. Motlhanka, K. Lebani, T, Boekhout, N. Zhou, “Fermentative Microbes of Khadi, a Traditional Alcoholic Beverage of Botswana,” Fermentation, vol. 6, no. 2, pp. 51, 2020.
     Google Scholar
  2. G. Aboagye, S. Gbolonyo-Cass, N. K. Kortei, T. Annan, “Microbial evaluation and some proposed good manufacturing practices of locally prepared malted corn drink (“asaana”) and Hibiscus sabdariffa calyxes extract (“sobolo”) beverages sold at a university cafeteria in Ghana,” Scientific African, e00330, 2020.
     Google Scholar
  3. R. Kubo, S. Funakawa, S. Araki, N. Kitabatake, “Production of indigenous alcoholic beverages in a rural village of Cameroon,” Journal of the Institute of Brewing, vol. 120, no. 2, pp. 133-141, 2014.
     Google Scholar
  4. J. N. Katongole, “The microbial succession in indigenous fermented maize products,” Ph.D. thesis, University of the Free State, Bloemfontein. South Africa, 2008.
     Google Scholar
  5. J. De Roos, L. De Vuyst, “Microbial acidification, alcoholization, and aroma production during spontaneous lambic beer production,” Journal of the Science of Food and Agriculture, vol. 99, no. 1, pp. 25-38, 2019.
     Google Scholar
  6. S. Aka, G. Konan, G. Fokou, K.M. Dje, B. Bonfoh, “Review on African traditional cereal beverages,” Am. J. Res. Commun., vol. 2, no. 5, pp. 103-153, 2014.
     Google Scholar
  7. K. Motlhanka, N, Zhou, K. Lebani, “Microbial and chemical diversity of traditional non-cereal based alcoholic beverages of Sub-Saharan Africa,” Beverages, vol. 4, no. 2, p. 36, 2018.
     Google Scholar
  8. M. Pauline, O. Alexandre, B.K. Andoseh, M. T. S. Abeline, T. Agatha, “Production technique and sensory evaluation of traditional alcoholic beverage based maize and banana,” International journal of gastronomy and food science, vol. 10, pp. 11-15, 2017.
     Google Scholar
  9. W. E. van Beek, “Kapsiki beer dynamics,” in Food resources and food choices in the Lake Chad basin, IRD, Paris, 2006, pp. 477-500.
     Google Scholar
  10. J. R. Bayoï, D. R. Darman, F-X. Etoa, “Technologie de fabrication, propriétés physico-chimiques et microbiologiques de la bière kapsiki blanche produite dans les monts Mandara au Nord – Cameroun,” Afrique Sciences, vol. 12, pp. 123-134, 2016.
     Google Scholar
  11. J. R. Bayoï, D. R. Darman, “Traditional processing and quality control of the “red kapsiki”: a local sorghum beer from northern Cameroon,” in Brewing Technology, chap. 8, published by Intech, England, 2017, pp. 159-175.
     Google Scholar
  12. S. A. Kim, S. H. Jeon, N. H. Kim, H. W. Kim, N. Y. Lee, T. J. Cho and others, “Changes in the microbial composition of microbrewed beer during the process in the actual manufacturing line,” Journal of food protection, vol. 78, no. 12, pp. 2233-2239, 2015.
     Google Scholar
  13. S. U. Oranusia, V. J. Umoha, J. K. P. Kwagab, “Hazards and critical control points of kunun-zaki, a non-alcoholic beverage in Northern Nigeria,” Food Microbiology, vol. 20, pp. 127–132, 2003.
     Google Scholar
  14. ISO 4833:2003, “ Méthode horizontale pour le dénombrement des microorganismes-Technique de comptage des colonies à 30°C”, in Microbiologie des Aliments, International Standards Organization, Geneva, Switzerland, 2003.
     Google Scholar
  15. ISO 21527-2:2008, “ Méthode horizontale pour le dénombrement des levures et moisissures- Partie 1: Technique par comptage des colonies dans les produits à activité d'eau supérieure à 0.95”, in Microbiologie des Aliments, International Standards Organization, Geneva, Switzerland, 2008.
     Google Scholar
  16. ISO 4832:2006, “Méthode horizontale pour le dénombrement des coliformes-Méthode par comptage des colonies”, in Microbiologie des Aliments, International Standards Organization, Geneva, Switzerland, 2006.
     Google Scholar
  17. ISO 9308-1:2000, “ Recherche et dénombrement des Escherichia coli et des bactéries coliformes - Partie 1: Méthode par filtration sur membrane”, in Microbiologie des Aliments, International Standards Organization, Geneva, Switzerland, 2000.
     Google Scholar
  18. SP-VG M008 (3) :1998, “Méthode de dénombrement des spores de microorganismes aérobies (méthode de routine par comptage des colonies à 30°C) ”, in Microbiologie des Aliments, Santé Publique – Volksgezondheid, Bruxelles, Belgique. 1998.
     Google Scholar
  19. N. Maoura, M. Mbaiguinam, H. V. Nguyen, C. Gaillardin, J. Pourquie, “Identification and typing of yeast strains isolated from bili-bili, a traditional sorghum beer of Chad,” African Journal of Biotechnology, vol. 4, no. 7, pp. 646-656, 2006.
     Google Scholar
  20. L. J Lima, H. J. Kamphuis, M. J. Nout, M. H. Zwietering, “Microbiota of cocoa powder with particular reference to aerobic thermoresistant spore-formers,” Food Microbiology, vol. 28, pp. 573–582, 2011.
     Google Scholar
  21. F. Postollec, A. G. Mathot, M. Bernard, M. L. Divanac'h, S. Pavan, D. Sohier, “Tracking spore-forming bacteria in food: from natural biodiversity to selection by processes,” International Journal of Food Microbiology, vol. 158, pp. 1–8, 2012.
     Google Scholar
  22. S. A. Scott, J. D. Brooks, J. Rakonjac, K. M. Walker, S. H. Flint, “The formation of thermophilic spores during the manufacture of whole milk powder,” International Journal of Dairy Technology, vol. 60, pp. 109-117, 2007.
     Google Scholar
  23. E. Baril, “Quantification de l'influence de l’environnement sur la formation et la thermorésistance des spores bactériennes,” Ph.D. thesis, Université de Bretagne Occidentale, Brest, France, 2011.
     Google Scholar
  24. J. C. Augustin, “Challenges in risk assessment and predictive microbiology of foodborne spore-forming bacteria,” Food Microbiology, vol. 28, pp. 209-213, 2011.
     Google Scholar
  25. M. Heyndrickx, “The importance of endospore-forming bacteria originating from soil for contamination of industrial food processing,” Appl. Environ. Soil Sci., pp. 1–11, 2011.
     Google Scholar
  26. M. Bartoszewicz, B. M. Hansen, I. Swiecicka, “The members of the Bacillus cereus group are commonly present contaminants of fresh and heat-treated milk,” Food Microbiology, vol. 25, pp. 588-596, 2008.
     Google Scholar
  27. S. J. Oomes, A. van Zuijlen, J.O. Hehenkamp, H. Witsenboer, J. Van der Vossen, S. Brul, “The characterisation of Bacillus spores occurring in the manufacturing of (low acid) canned products,” International Journal of Food Microbiology, vol. 120, 85-94, 2007.
     Google Scholar
  28. S. A. Burgess, D. Lindsay, S. H. Flint, “Thermophilic bacilli and their importance in dairy processing,” International Journal of Food Microbiology, vol. 144, 215-225, 2010.
     Google Scholar
  29. F. A. Oguntoyinbo, “Development of hazard analysis critical control points (HACCP) and enhancement of microbial safety quality during production of fermented legume based condiments in Nigeria,” Nigerian Food Journal, vol. 30, no. 1, pp. 59-66, 2012.
     Google Scholar
  30. W. H. Holzapfel, “Appropriate starter culture technologies for small-scale fermentation in developing countries,” International Journal of Food Microbiology, vol. 75, pp. 197-212, 2002.
     Google Scholar
  31. L. Jespersen, “Occurrence and taxonomic characteristics of strains of Saccharomyces cerevisiae predominant in African indigenous fermented foods and beverages,” Yeast Research, vol. 3, pp. 191-200, 2003.
     Google Scholar
  32. M. K. Djè, S. Aka, Y. Z. Nanga, K. C. Yao, Y. G. Loukou, “Predominant lactic acid bacteria involved in the spontaneous fermentation step of tchapalo process, a traditional sorghum beer of Côte d’Ivoire,” Research Journal of Biological Sciences, vol. 4, no. 7, pp. 789-795, 2009.
     Google Scholar
  33. C. L. Ramos, E. G. de Almeida, G. V. de Melo Pereira, P. G. Cardoso, E. S. Dias, R. F. Schwan, “Determination of dynamic characteristics of microbiota in a fermented beverage produced by Brazilian Amerindians using culture-dependent and culture-independent methods,” International Journal of Food Microbiology, vol. 140, no. 2, pp. 225-231, 2010.
     Google Scholar
  34. S. Sefa-Dedeh, A.I. Sanni, G. Tetteh, D. E. Sakyi, “Yeasts in the traditional brewing of pito in Ghana,” World Journal of Microbiology and Biotechnology, vol. 15, no. 5, pp. 983-997, 1999.
     Google Scholar
  35. A. P. P. Kayodé, G. Vieira-Dalodé, A. R. Linnemann, S. O. Kotchoni, A. J. D. Hounhouigan, M. van Boeke, M. J. R. Nout, “Diversity of yeasts involved in the fermentation of tchoukoutou, an opaque sorghum beer from Benin,” African Journal of Microbiology Research, vol. 5, no. 18, pp. 2737-2742, 2011.
     Google Scholar
  36. M. M. Coman, M. C. Verdenelli, C. Cecchini, S. Silvi, C. Orpianesi, N. Boyko, A. Cresci, “In vitro evaluation of antimicrobial activity of Lactobacillus rhamnosus IMC 501, Lactobacillus paracasei IMC 502 and SYNBIO against pathogens,” Journal of Applied Microbiology, vol. 117, no. 2, pp. 518-527, 2014.
     Google Scholar
  37. R. D. Darman, J. J. Essia Ngang, F-X. Etoa, “Amelioration of cassava retting by a starter culture of three strains,” International Journal of Innovation and Scientific Research, vol. 14, no. 2, pp. 268–277, 2015.
     Google Scholar
  38. J. G. LeBlanca, S. D. Todorov, “Bacteriocin producing lactic acid bacteria isolated from Boza. a traditional fermented beverage from Balkan Peninsula,”A. Méndez Vilas, Communicating current research and technological advances” pp. 1311-1320, 2011.
     Google Scholar
  39. H. C. Okereke, O. K. Achi, U. N. Ekwenye, F. A. Orji, “Antimicrobial properties of probiotic bacteria from various sources,” African Journal of Biotechnology, vol. 39, no. 11, pp. 9416-9421, 2012.
     Google Scholar


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