Ameliorative Effects of Pseudomonas fluorescence Strains on Growth and Antioxidant Potential of Okra (Abelmoschus esculentus) Plant under Nematode Infection

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  •   A. M. Esan

  •   Z. Khan

  •   R. Kiran

  •   T. O. Omolekan

  •   K. A. Aremu

  •   H. R. Y. Adeyemi

Abstract

Meloidogyne incognita is a plant pathogen causing root-knot nematodes disease in many crops worldwide. Due to the environmental threat on the use of chemical fumigants, there is a need for a biological control method using microbial antagonists on root-knot nematodes disease. Therefore, this study was conducted to screen and evaluate the biocontrol potential of P. fluorescens strains against root-knot nematodes. The effectiveness of six P. fluorescens strains viz., Pf1, Pf2, Pf3, Pf4, Pf5and Pf6 were tested in vitro and also in pots experiment for their inhibitory activities and biocontrol potential against root-knot nematodes disease caused by Meloidogyne incognita on okra plant. Treatments of the nematode with 1.0-6.0% concentrations of 108 CFU/mL of Pf4 and Pf5 strains caused 70.0-95.0% inhibition on nematode egg-hatch and 2nd stage juveniles activity. Pf3, Pf4 and Pf5showed a decrease in the number of roots galling with increased root and shoot dry weights of stressed okra plant. Moreover, there was 25.99-36.43%, 37.76-79.145% and 42.62-62.37%, 69.83-98.09% increase in shoot length and leaf areas after 15th and 30th day respectively of P. fluorescens inoculation. The inoculated okra plants exhibited higher photosynthetic pigments, higher antioxidant enzymes activity and mineral contents than the nematode treated groups. Higher mineral contents were observed in the roots than the leaves of the okra plant subjected to the nematode infection. The bacteria strains especially Pf4 and Pf3 have considerable potential to reduce the menace of the nematodes in the treated okra plant. Therefore, the strains can be used for crop management against root-knot nematodes disease.


Keywords: Antioxidant enzymes, Meloidogyne incognita, Minerals, Okra, Pseudomonas fluorescens

References

D. M. Weller, J. M. Raajmakers, B. B. Gardener, L. S. Thomashow, Microbial populations responsible for specific soil suppressiveness to plant pathogens. Annu. Rev. Plant Pathol. 40, 309-348, 2002.

D. J. Hunt, Z. A. Handoo, Taxonomy, identification and principal species. In: Root-knot nematodes (Eds. Perry R. N., Moens M. and J. L. Starr). CAB International, Wallingford, UK., pp. 55-97, 2009.

M. Moens, R. N. Perry, J. L. Starr, Meloidogyne species a diverse group of novel important plant parasites. p.1–17. In: “Root-Knot Nematodes” (R.N. Perry, M. Moens, J.L. Starr, eds.). CABI Publishing, Wallingford, Oxon, UK, pp. 488, 2009.

H. Melakeberhan, J. M. Webster, Brooke RC, D’Auria JM and Cackette M, Effects of Meloidogyne incognita on plant nutrient concentration and its influence on the physiology of beans. Journal of Nematology, 19, 324-330, 1987.

A. D. M. Glass, Biological roles of the inorganic elements. In: Plant nutrition, an introduction to current concepts. Boston, MA: Jones and Bartlett Publishers. pp. 163-202, 1989.

K. D. Devrajan, G. Rajendran, N. Seenivasan, Nutrient status and photosynthetic efficiency of banana (Musa spp.) influenced by Meloidogyne incognita infected with Pratylenchus penetrans. Nematologia Mediterranea, 3, 197-200, 2003.

K. R. Kranthi, D. R. Jadhav, S. Kranthi, Wanjari RR, Ali SS and Russell DA, Insecticide resistance in five major insect pests of cotton in India. Crop Protec. 3, 449-460, 2002.

M. Ahmad, M. I. Arif, Z. Ahmad, Resistance to carbamate insecticides in Helicoverpa armigera (Lepidoptera: Noctuidae) in Pakistan. J. Crop protect.10, 427-432, 2001.

M. W. Abbasi, N. Ahmed, M. J. Zaki, S. S. Shaukat, Effect of Barleria acanthoides Vahl. on root-knot nematode infection and growth of infested okra and brinjal plants. Pakistan Journal of Bot. 40, 2193-2198, 2008.

M. M. M. Abd-Elgawad, The current status of phytonematode management in Egypt with special reference to applicable nematicides. Egyptian J. Agronemato 6, 33-46, 2008.

Y. Huang, C. Xu, L. Ma, K. Zhang, C. Duan, M. Mo, Characterization of volatiles produced from Bacillus megaterium YFM 3.25 and their nematicidal activity against Meloidogyne incognita. European J. Plant Pathol. 26, 417-422, 2009.

C. L. Patten, B. Glick, Bacterial biosynthesis of indole-3-acetic acid. Can. J. Microbiol., 42, 207-220, 1996.

K. A. Abo-Elyousr, Z. Khan, M. E. Awad, M. F. Abdel-Moneim, Evaluation of plant extracts and Pseudomonas spp. for control of root-knot nematode, Meloidogyne incognita on tomato. Nematropica, 40, 289-300, 2010.

J. F. Southey, Laboratory methods for work with plant and soil nematodes. Min. Agric. Fish. Food. HMSO, London, U.K. pp. 202, 1986.

D. Sukran, T. Gunes, R. Sivac, Spectrophotometric determination of chlorophyll-A, B and carotenoid contents of some Algae species using different solvents. Tr. J. of Botany. 22, 13-17, 1998.

M. L. Jackson, Soil chemical analysis. Prentice-Hall of India, New Delhi, pp. 498, 1973.

L. Jorhem, Determination of metals in foodstuffs by atomic absorption spectrophotometry after dry ashing. NMKL Interlaboratory study of lead, cadmium, zinc, copper, iron, chromium and nickel. J. AOAC International, 76, 798-813, 1993.

E. Esfandiari, M. R. Shakiba, S. Mahboob, H. Alyari, M. Toorchi, Water stress, antioxidant enzyme activity, and lipid peroxidation the wheat seedling. J. Food Agric. Environ. 5, 149-153, 2007.

J. T. Rotruck, A. L. Pope, H. E. Ganther, A. B. Swanson, D. G. Hafeman, W. G. Hoekstra, Selenium: Biochemical roles as a component of glutathione peroxidase. Science, 179, 588-590, 1984.

R. S. Dhindsa, P. Plumb-Dhindsa, T. A. Thorpe, Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase J. Exp. Botany. 32, 93-101, 1981.

K. Chen, K. Gunte, M. D. Maines, Neurons overexpressing heme oxygenase-1 resist oxidative stress-mediated cell death. J. Neuro chem. 75, 304-313, 2000.

K. A. A. Elyousr, Z. Khan, E. M. M. Award, M. F. A. Moneim, Evaluation of plant extracts Pseudomonas spp. for control of root-knot nematode, Meloidogyne incognita on tomato, Nematropica., 40(2), 288-299, 2010.

B. Anita, G. Rajendran, R. Samiyappan, Induction of systemic resistance in tomato against root-knot nematode, Meloidogyne incognita by Pseudomonas fluorescens. Nematol. Medit. 32 (1), 47-51, 2004.

C. Shaukat, P. P. K. Chahal, Effect of thuricide on the hatching of eggs root-knot nematode, Meloidogyne incognita. Current Nematol., 3(1), 41-46, 2002.

J. San, S. S. Shaukat, Biocontrol activity of Rhizobacteria active against plant parasitic nematodes. J. Biofert. Biopest., pp: 54-59, 2009.

A. E. Richardson, Prospects for using soil microorganisms to improve the acquisition of phosphorus by plants. Austral J. of Plant Physiol. 28, 897-906, 2001.

C. L. Patten, B. R. Glick, Role of Pseudomonas putida indoleacetic acid in the development of the host plant root system. Appl. and Environ. Microbiol. 68, 3795-3801, 2002.

I. A. Siddiqui, S. Shaukat, Systemic resistance in tomato induced by biocontrol bacteria against root-knot nematode, Meloidogyne javanica is independent of salicylic acid production. Journal of Phytopathol. 152, 48-54, 2004.

P. J. Davies, The Plant Hormones: Their Nature, Occurrence, and Functions in P. J. Davies, Eds. Plant Hormones and Their Role in Plant Growth and Development. pp. 1-11, 1987.

S. Karpinski, H. Gabrys, A. Mateo, B. Karpinska, P. M. Mullineaux, Light perception in plant disease defence signalling. Current Opinion in Plant Biology, 6, 390-396, 2003.

H. Xu, W. B. Ruan, Y. B. Gao, X. Y. Song, Y. K. Wei, Effects of root-knot nematodes on cucumber leaf N and P contents, soil pH and soil enzyme activities. Ying Yong Sheng Tai Xue Bao 21, 2038-2044, 2010.

D. H. Lee, Y. Kim, C. B. Lee, The inductive responses of the antioxidant enzymes by salt stress in the rice (Oryza sativa L.). J. Plant Physiol. 158, 737-745, 2001.

R. Mittler, Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7, 405-410, 2002.

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How to Cite
Esan, A. M., Khan, Z., Kiran, R., Omolekan, T. O., Aremu, K. A., & Adeyemi, H. R. Y. (2021). Ameliorative Effects of Pseudomonas fluorescence Strains on Growth and Antioxidant Potential of Okra (Abelmoschus esculentus) Plant under Nematode Infection. European Journal of Biology and Biotechnology, 2(3), 50-56. https://doi.org/10.24018/ejbio.2021.2.3.168