Recombinant Sip Young 6 His Protein Production from the Sea Star Igkappa Gene, after Cloning



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Submitted Mar 20, 2021
Published Apr 12, 2021
10.24018/ejbio.2021.2.2.175

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In 2014 we have isolated and cloned the sea star Igkappa gene which induced an anti-HRP primitive invertebrate antibody. We attempt, in the present work, for the first time in Invertebrates, to produce a SIP Young 6 His protein through HeK 293 EBNA cells.

Keywords: Invertebrates; HEK 293 cells; sea star Igkappa gene; Asterias rubens

References

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