Blood Glucose – Lowering Potentials of Annona muricata Leaf Extract in Alloxan–Induced Diabetic Rats



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Submitted Mar 25, 2021
Published Apr 29, 2021
10.24018/ejbio.2021.2.2.178

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The blood glucose- lowering potentials of ethanol leaf extract of Annona muricata were studied. Thirty wistar albino rats were divided into six groups of five rats per group. Group 1 served as “Normal control” animals and received normal rat pellets and water. Diabetes mellitus was induced in Groups 2, 3, 4, and 5 by intraperitoneal injection of alloxan (130 mg/kg). Group 6 rats were administered with 400 mg/kg daily of the extract without induction; group 3 rats were treated with glibenclamide (5 mg/kg body weight), groups 4 and 5 received 200 mg/kg and 400 mg/kg body weight of A. muricata leaf extract daily respectively throughout the duration of the experiment of 14 days. Group 2 rats were induced but not treated with any drug, thus it served as the “Negative control” group. Quantitative phytochemical analysis of the leaf extract was carried out using the Association of Official Analytical Chemists (AOAC) methods. Acute toxicity test of the leaf extract of A. muricata was determined using 12 rats by Lorke’s toxicity testing method. The blood glucose levels of the animals in each group were determined using Accu-chek test strip method. The weights of the animals were determined using a standard electronic weighing balance. The result of the quantitative phytochemical analysis of the leaf showed that the ethanol leaf extract contains the following: phenols (74 mg/100 g), flavonoids (3.70 mg/100 g), tannins (2.95 mg/100 g), oxalate (6.48 mg/100 g), terpenoid (13.88 mg/100 g), phytates (130 mg/100 g), saponins (6800 mg/100 g), alkaloids (570 mg/100 g), cardiac glycoside (1690 mg/100g). Acute toxicity studies showed that LD50 was 3807.89 mg/kg body weight. The results of the average blood glucose levels (mg/dl) of the rats in each group were group 1, 82.6071±7.7524, group 2, 309.3571±163.6923, group 3, 226.7143±132.8182, group 4, 146.5000±140.1465, group 5, 150.4783±81.8340, and group 6, 83.4643±12.5329 for each group respectively. The average body weights of the rats in each group were group 1, 192.8571±22.5844, group 2, 185.7143±33.6759, group 3, 177.1429±36.67500, group 4, 219.2857±21.2908, group 5, 119.5455±23.5993, and group 6, 191.7857±25.2475. The findings from this study suggest that ethanolic leaf extract of A. muricata has notable effect in lowering blood glucose levels in diabetic rats and is a more potent drug in the treatment and management of diabetes mellitus and oxidative stress- related diseases.

Keywords: Annona muricata, Acute Toxicity testing, Blood glucose-lowering, Quantitative phytochemicals

References

  1. Abdullah, I.A., Azbo, M.O., Amos, S.G., Gamaniel, K.S. and Wambebe, C., (2001) Antidiabetic Activity of the Aqueous extract of Nauclea latifolia root. Phytother Resources. 52:431-434.
     Google Scholar
  2. Adewole, S. and Ojewole, J. (2009) Protective effects of Annona murcata Linn (Annonaceae) leaf aqueous extract on serum lipid profile and oxidative stress in hepatocytes of streptozotocin-treated diabetic rats. African Journal of Traditional, Complementary and Alternative Medicines, 6: 30-41.
     Google Scholar
  3. Adeyemi, D.O., Komolafe, O.A., Adewole, O.S., Obuotor, E.M. and Adenowo, T.K. (2009) Anti hyperglycemic activities of Annona muricata (Linn). African Journal of Traditional Complements and Alternative Medicine. 6:62–69.
     Google Scholar
  4. Anonymous 1, (2017) Oxalate, https://en.wikipedia.org/wiki/Oxalate accessed 12/5/2017.
     Google Scholar
  5. Anonymous 2, (2017) Phytic acid, https://en.wikipedia.org/wiki/Phytic_acid accessed 12/5/2017.
     Google Scholar
  6. Aroma, D. and Enegide, C. (2014) The Importance of Toxicity Testing. Journal of Pharmaceutical and Biosciences, 4(2013): 146-148.
     Google Scholar
  7. Arthur, F.K.N, Woode, E., Terlabi, E.O. and Larbie, C. (2011) Evaluation of acute and sub-chronic toxicity of Annona muricata (Linn) aqueous extract in animals. European Journal of Experimental Biology 1 (4): 115-124.
     Google Scholar
  8. Barros, L., Ferreira, M.J., Queiros, B., Ferreira, I. C. F. R and Batista, P. (2007). Total phenols, ascorbic acid, á-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities, Food Chemistry,103:1314-419.
     Google Scholar
  9. Coria-Tellez, A.V., Montalvo-Gonzalez, E., Yahia, M.E. and Obiedo-Vazquez, E.N. (2016), Annona muricata: A comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity. Arabian Journal of Chemistry. 1:1-2.
     Google Scholar
  10. Edeoga, H.O., Okwu, D.E. and Mbaebie, B.O. (2005) Phytochemical Constituents of some Nigeria medicinal plants. Africa Journal of Biotechnology. 4(7): 685-688.
     Google Scholar
  11. Etuk, E.U. (2010) Animal Models for studying Diabetes Mellitus. Agriculture and Biology Journal of North America, 1(2):130-134.
     Google Scholar
  12. Gaikwad, S.B, Mohen, G.K and Rani, S.M (2014) Phytochemicals for diabetes management. pharmaceutical crops, 5(supp1:M2)11-28.
     Google Scholar
  13. Gotep, J.G., Agada, G.O.A., Gbisi, D.S. and Chollom, S., (2010) Antibacterial Activitiy of Ethanolic extract of Acalypha wilkesiana leaves growing in Jos, Plateau State, Nigeria. Malaysian Journal of Microbiology 6(2):69-74.
     Google Scholar
  14. Guclu-Ustundag O. and Mazza G. (2007) Saponins: Properties, Applications and Processing. Critical Reviews in Food Science and Nutrition, 47:231-258.
     Google Scholar
  15. Harborne, J.B. (1973) Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis (Third Edition). Chapman and Hall, New York. Pp 674-681.
     Google Scholar
  16. Just, M.J, Recio, MC, Giner, R.M, Cuellar, M.J, Manez S., Bilia A.R. and Rios J.L., (1998) Anti-inflammatory activity of unusual lupine saponins from Bupleurum fruticescens. Journal of Medicinal Plants. 64: 04-407.
     Google Scholar
  17. Kabera J.N., Semana, E., Mussa, A.R. and He, X. (2014) Plant Secondary Metabolites: Biosynthesis, Classification, Function and Pharmacological Properties. Journal of Pharmacy and Pharmacology, 2: 377-392.
     Google Scholar
  18. Lorke, D. (1983) A new Approach to practical Acute Toxicity Testing. Archives of Toxicology, 54:275-287.
     Google Scholar
  19. Lukacinova, A., Mojzis, J., Benacka, R., Keller, J., Maguth, T., Kurila, P., Vasko, L., Racz, O., and Nistiar, F. (2008) Preventive effects of flavonoids on Alloxan-induced Diabetes mellitus in Rats. Acta VeterinariaBrunensis, 77:175-182.
     Google Scholar
  20. McDevitt J.T., Schneider D.M., Katiyar S.K. and Edlind T.D., (1998) Berberina: A candidate for the treatment of diarrhea in AIDS patients abstract 175. In Program and Abstract of the 36thInterscience Conference on Antimicrobial Agents and Chemotherapy. American Society forMicrobiology. Washington D.C.
     Google Scholar
  21. Moghadamtousi, S.Z., Fadaeinasab M., Nikzad S., Mohan G., Ali H.M. and Kadir H.A., (2015) Annona Muricata (Annonaceae): A Review of its traditional uses, isolated Acetogenins and Biological Activities. International Journal of Molecular Science, 16:15625-15658.
     Google Scholar
  22. Moghadamtousi, S.Z., Goh B.H., Chan C.K., Shabab T. and Kadir H.A., (2013) Biological activities and Phytochemcials of Swietenia macrophylla.King Molecule 18:10465-10483.
     Google Scholar
  23. Narayan, G., Sondipon C., Shamik G., Samir K. S. and Suman B. G. (2016) Terpenoids concentration in plant tissues using a monoterpene, Linalool as standard reagent. Laboratory, West Bengal State University. 64:3-19.
     Google Scholar
  24. Okwu, D.E. (2004) Phytochemicals and vitamin content of indigenous species of southeastern Nigeria. Journal of Sustainable Agriculture and the Environment, 6(1): 30-37.
     Google Scholar
  25. Orwa, C., Mutua A., Kindt R., Jamnadass R. and Anthony S., (2009) Agroforest tree Database: 9 free reference and selection guide version 4.0 (http://www.worldagroforestory.org/sites/tredbs/treedatabases.asp).
     Google Scholar
  26. Osagie, A.U. (1998) Antinutritional Factors In: Nutritional Quality of Plant Foods. Ambik Press Ltd, Benin City, Nigeria. Pp. 1-40; 221-244.
     Google Scholar
  27. Porter, J.R. and Barrett, T.G. (2005) Monogenic Syndromes of abnormal glucose homeostasis: Chemical review and relevance to the understanding of the pathology on insulin resistance and beta cell failure. Journal of Medical Genetics, 42(12):893-903.
     Google Scholar
  28. Ruch, R.J., Cheng, S.J. and Klaunig, J.E. (1989) Prevention of cytotoxicity and inhibition of Intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogens, 10:1003-1008.
     Google Scholar
  29. Sulaiman, H., Roslida, A.H., Fezah, O., Tan, K.L., Tor, Y.S. and Tan, C.I. (2012) Chemopreventive Potential of Annona muricata Linn Leaves on Chemically-Induced Skin Papillomagenesis in Mice. Asian Pacific Journal for Cancer Prevention, 13: 2533-2539.
     Google Scholar
  30. Trease, G.W and Evans, W.C. (2005), Alkaloids in Evans W.c., (editor), Pharmacognocy. 15th (edition), New Delhli, India. Pp 333-338.
     Google Scholar
  31. Trupti, P.S. and Dayamand, P.G. (2014) A Brief Review on Recent Advances in Chemical Research of Annonamuricata. International Journal of Universal Pharmacy and Bioscience, 3(3): 267-303.
     Google Scholar
  32. Usunobun, U., and Okolie, P.N., (2015) Phytochemical Analysis and Mineral Composition of Annonamuricata leaves. International Journal of Research and Curremnt Development. 1(1): 38-42.
     Google Scholar
  33. Usunobun, U., Okolie, N.P., Anyanwu, O.G., Adegbeg, A.J. and Egharevba, M.E. (2015) Phytochemical Screening and Proximate Composition of Annonamuricata leaves. European Journal of Botany Plant Science and Phytology, 2(1) 18-28.
     Google Scholar
  34. Wheeler, E.L. and Ferrel, R.E. (1971) A Method for Phytic Acid Determination in Wheat and Wheat Fractions. Cereal Chemistry journal, 48:312-320.
     Google Scholar
  35. World Health Organization, (2008), Fact sheet No. 134. Traditional Medicine (http.//www.who.int). accessed 10/5/2017.
     Google Scholar
  36. Yukari, I., Youichi, F., Ikuko, N., Itsuru, Y. (1995) Quantitative HPLC analysis of cardiac glycosides in Digitalis purpurea leaves. Journal of Natural Products, 58(60):897-901.
     Google Scholar