Microscopic and Molecular Detection of Entamoeba histolytica in Selected Public Primary School Pupils of Bwari Area Council, F.C.T Abuja, Nigeria


  •   F. R. Okusaga

  •   R. J. Ombugadu

  •   A. B. Yako

  •   G. A. Amuga


This study evaluated the prevalence of Entamoeba histolytica using microscopic detection and confirmed by DNA analysis using polymerase chain reaction (PCR) technique by taking samples of some public primary school pupils within Bwari Area Council of Abuja, Nigeria. Microscopic and polymerase chain reaction-restriction fragment length polymorphism analysis targeting only a small portion (228 bp) of the adh112 gene. Results showed that out of three hundred and twenty (320) stool sample screened, twenty six 26 (8.1%) were found to be positive of the parasite. The prevalence was high among pupils in school 4 with 7 (11.2%) follow by school 5 with (11.1%), school 3 with 5(8.3%), school 1 with 5(7.3%) and the lowest school 2 with (2.9%) P<0.05. The prevalence was high among male with 18 (13.2%) than the female P<0.05. The prevalent was high among age 4-9 had more E. histolytica than other age group with 16 (12.8%) P<0.05. The prevalence of E. histolytica was high among those that use Open space system toilet with 11 (14.4%) than the other risk factors examined P<0.05. The polymerase chain reaction-restriction fragment length polymorphism showed that the entire twenty six parasite were E. histolytica as given on the Agarose gel electrophoresis with adh112 gene band of 228bp. The sources of contracting amoebiasis include drinking of contaminated water, poor sanitation in living quarters, lack of or improper use of toilets, contact with flies and items contaminated by flies, among other hygiene related issues.

Keywords: Entamoeba histolytica, hygiene, prevalence, pupils


Reuben, C. R., Katsa, M., Hassan, S. C. (2013). Prevalence of intestinal amoebiasis in school age children in Lafia, Nassarawa State, Nigeria. International Research Journal of Biological Sciences, 2(7): 42-45.

Simon-Oke, I. A. and Ogunleye, E. (2015). Prevalence of Entamoeba histolytica among primary school children in Akure, Ondo State, Nigeria. Journal of Public Health and Epidemiology, 7(11): 346351.

Nader, P., Mahnaz, N., Ali, H., Mansour, S. and Hosinali, Y. (2015). Frequency of Entamoeba histolytica and Entamoeba dispar prevalence among patients with gastrointestinal complaints in Chelgerd city, Southwest of Iran. Journal of Medical Science Research. 16(11): 1436- 1440.

Amaechi, E. C., Ohaeri, C. C., Ukpai, O. M., Nwachukwu, P. C. and Ukoha, U.K. (2014). Prevalence of Entamoeba histolytica among primary school children in Ukwa West Local Government Area, Abia State, South East, Nigeria. The Bioscientist, 2(1): 1-7.

Fotedar, R., Stark, D., Beebe, N., Marriott, D., Ellis, J.and Harkness, J. (2007). Laboratory diagnostic techniques for Entamoeba species. Clinical Microbiology Revision. 20(3): 32-40.

Gimba, U. N., Azare, A. B. and Alao, A. O. (2014). Evaluation of Entamoeba histolytica transmission rates amongst primary school children in five selected communities in Gwagwalada Area council, FCT-Abuja, Nigeria. Journal of Biology, Agriculture and Healthcare, 4(27): 22-30.

Federal Capital Development Authority (2018). Planning Research Corporation geographical layout.

Kombo, D. and Tromp, D. (2016). Proposal and thesis writing, an introduction. Paulines Publications, Nairobi, Kenya, 2: 70-79, 130-133.

Wayne, W. (2010). Biostatistics; Basic concepts and methodology for the Health Sciences,9th Edition. Willey and Sons, Asia. Pp. 192-196.

Cheesbrough M. (2009). District laboratory practice in tropical countries. Part 1.6th Ed. Cambridge University Press. 350.

Saeed, A. A and Manal, B. J (2007). Diagnosis and differentiation of Entamoeba infection in Makkah Al Mukarramah using microscopy and stool antigen detection kits. World Journal of Medical Science. 2(1): 15-20.

Ngobeni, R., Abhyankar, M. M., and Jiang, N. M. (2017). Entamoeba histolytica-encoded homolog of macrophage migration inhibitory factor contributes to mucosal inflammation during amebic colitis. Journal of Infectionus Diseases; 215: 1294 –302.

Mortimer, L., Moreau, F., Cornick, S., and Chadee, K. (2014). Gal-lectin-dependent contact activates the inflammasome by invasive Entamoeba histolytica. Mucosal Immunol. 7, 829–841.

Inabo, H., Galadima, M., Ogbadu, L. J and Okuofu, C. A (2000). Prevalence of Entamoeba histolytica and Giardia lambliain primary school pupils in five rural villages around Kaduna and Zaria, Nigeria. Nigerian Journal of Parasitology. 21: 61-67.

Aza, N., Ashley S and Albert, J. (2003). Parasitic Infections in human communities living on the fringes of the Crocker range park, Sabah, Malaysia. ASEAN Revision of Biodiversity and Environmental Conservation (ARBEC). 3: 100-110.

Aksoy, U., Akisu, C., Bayram-Delibas, S., Ozkoc, S and Sahin, S, (2007). Demographic status and prevalence of intestinal parasitic infections in school children in Izmir, Turkey. Turkish Journal of Paediatrics. 49: 278-282.

Ozlem, O and Hakan, S. (2005). The contamination of various fruits and vegetables with Eterobius vemicularis, Ascaris eggs, E. histolytica cysts and Giardia cysts. Science Direct, 16(6): 557-560.


How to Cite
Okusaga, F. R., Ombugadu, R. J., Yako, A. B., & Amuga, G. A. (2021). Microscopic and Molecular Detection of Entamoeba histolytica in Selected Public Primary School Pupils of Bwari Area Council, F.C.T Abuja, Nigeria. European Journal of Biology and Biotechnology, 2(3), 57-60. https://doi.org/10.24018/ejbio.2021.2.3.196