Growth Optimization of Glyphosate-based Herbicides Utilizing Bacteria isolated from Lotic Water of Ogini Stream, Nigeria

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  •   R. E. Aso

  •   C. Hammuel

  •   M. Daji

  •   J. Briska

Abstract

Glyphosate-based herbicides are often used for the control of weeds grown on agricultural fields or farms. Different health problems have been reported to be associated with the use of glyphosate-based herbicides mainly due to their toxicity level. Thus, finding glyphosate utilizing microorganisms to remediate the glyphosate-based herbicides in the environment is crucial. The culture conditions for maximum utilization of glyphosate by bacterial isolates, Stenotrophomonas maltophilia, Bacillus cereus and Enterobacter aerogenes previously isolated from Ugini stream close to corn fields treated with glyphosate-based herbicide at Ofagbe, Delta State, Nigeria were optimized using mineral salt medium containing glyphosate as carbon source. The varied culture parameters assessed were temperature (30, 37 and 40 oC), pH (5, 6, 7, 8 and 9), initial glyphosate concentration (1, 3, 5, 7 and 9 g/L) and incubation time (2-14 days). Optical density (OD) at 560 nm of the culture was used to estimate cell growth or cell load of the glyphosate utilizing bacteria strains at every 2 days for 14 days. The following optimal conditions were determined: initial pH 9.0, incubation temperature 30 °C, initial concentration of glyphosate (1g/L) and incubation time of 12 days. Of the isolates on the medium containing the herbicide as sole carbon and energy source, Bacillus cereus showed the highest growth level (OD average, 0.127, pH average, 6.26. This was followed by Stenotrophomonas maltophilia (OD average = 0.114, pH average = 6.44) and Enterobacter aerogenes (OD average = 0.100, pH average, 6.56). At the increased of glyphosate in the medium there was decreased in growth of the bacteria. Bacillus cereus, Stenotrophomonas maltophilia and Enterobacter aerogenes indicated a high capacity to be able to degrade glyphosate. It is therefore concluded that the bacteria employed in this research can be recommended for bioremediation of environments contaminated with this chemical and further research should conducted to ascertain the catabolic genes present in these individual glyphosate degrading bacteria.


Keywords: cultivation conditions, glyphosate-degrading bacteria, optimization, water pollution

References

M.A. Wilson, and S.R. Carpenter, “Economic valuation of Freshwater ecosystem services in the United States”, Ecological Application. vol 9, pp. 772-783, 1999.

T.V. Shushkova, I.T. Ermakova, A.V. Sviridov, A.A. Leontievsky, “Biodegradation of glyphosate by soil bacteria: Optimization of cultivation and the method for active biomass storage”. Journal of Microbiology, 81:44-50, 2012.

U. Dorigo, C. Leboulanger, A. Bérard, A. Bouchez, J.F. Humbert and B. Montuelle, “Lotic biofilm community structure and pesticide tolerance along a contamination gradient in a vineyard area”. Aquatic Microbial Ecology, vol. 50, pp. 91-102, 2007.

J.E. Franz, M.K. Mao, and J.A. Sikorski, “Glyphosate: A Unique Global Herbicide”; American Chemical Society: Washington, DC, USA, pp. 521-527, 1997.

D.W. Kolpin, E.M. Thurman, E.A. Lee, M.T. Meyer, E.T. Furlong, and S.T. Glassmeyer, “Urban contributions of glyphosate and its degradate AMPA to streams in the United States”. Environmental Science and Technology, vol. 354, pp. 191-197, 2006.

A. Ghanem, P. Bados, A.R. Estaun, L.F. de Alencastro, S. Taibi, and J. Einhorn, “Concentrations and specific loads of glyphosate, diuron, atrazine, nonylphenol and metabolites thereof in French urban sewage sludge”. Chemosphere, vol. 69 pp.1368-1373, 2007.

F. Botta, G. Lavison, G. Couturier, F. Alliot, E. Moreau-Guigon, N. Fauchon, B. Guery, M. Chevreuil, and H. Blanchoud, “Transfer of glyphosate and its degradate AMPA to surface waters through urban sewerage systems”. Chemosphere. 77:133-139, 2009.

A. Carter, “How pesticides get into water and proposed reduction measures”. Pesticide Outlook, vol. 149, pp.15, 2000.

E. Mallat, and D. Barceló, “Analysis and degradation study of glyphosate and of aminomethylphosphonic acid in natural waters by means of polymeric and ion- exchange solid-phase extraction columns followed by ion chromatography-post column derivatization with fluorescence detection”. Journal of Chromatography, Vol. 823: 129-136, 1998.

E.A. Scribner, W.A. Battaglin, R.J. Gilliom, and M.T. Meyer, “Concentrations of glyphosate, its degradation product, aminomethylphosphonic acid, and glufosinate in ground- and surface-water, rainfall, and soil samples collected in the United States”, Scientific Investigations Report, U.S. Geological Survey, Reston, Virginia. pp 2007-5122, 2007.

I. Amoros, J.L. Alonso, S. Romaguera, and J.M. Carrasco, “Assessment of Toxicity of Glyphosate-based Formulation Using Bacterial Systems in water”. Chemosphere. vol. 67, pp. 2221-2228, 2007

A. Obojska, N.G. Ternana, B. Lejczak, P. Kafarski, and P. McMullan, “Organophosphate utilization by the thermophile Geobacillus caldoxylosilyticus T20”. Applied and Environmental Microbiology. vol. 68, pp. 2081-2084, 2002

R.H. Peng, Y.S. Tian, A.S. Xiong, W. Zhao, X.Y. Fu, H.J. Han, C. Chen, X.F. Jin, and Q.H. Yao, “A novel 5-enolpyruvylshikimate-3-phosphate synthase from Rahnella aquatilis with significantly reduced glyphosate sensitivity”. Journal of Biochemistry. 35: pp. 4199-210, 2012.

C.M. Liu, P.A. McLean, C.C Sookdeo, and F.C. Cannon,“Degradation of the herbicide glyphosate by members of the family Rhizobiaceae”. Archives of Microbiology, vol. 57, pp. 1799-1804.

Y.V. Kryuchkova, G.L. Burygin, N.E. Gogoleva, Y.V. Gogolev, M.P. “Chernyshova, O.E. Makarov, E.E. Fedorov, O.V. Turkovskaya, Isolation and characterization of a glyphosate-degrading Rhizosphere strain, Enterobacter cloacae K7”. Journal of Microbiological Research. 13: 34-37.

C.N. Ariole,P.O. Okerentugba, and R.E. Aso, “Isolation and molecular identification of glyphosate utilizing bacteria from lotic water”. Nigerian Journal Microbiology, vol. 30 pp. 2999-3006, 2015.

Ouided Benslama and Abderrahmane Boulahrouf, “solation and characterization of glyphosate-degrading bacteria from different soils of Algeria’’. Afr. J. Microbiol. Res. 7(49), pp. 5587-5595. 2013

A.N. Moneke, G.N. Okpala, and C.U. Anyanwu, “Biodegradation of glyphosate herbicide in vitro using bacterial isolates from four rice fields”. African Journal of Biotechnology. vol. 9, pp. 4067-4074, 2010.

V. Patel, S. Cheturvedula, D. Madamwar, “Phenanthrene degradation by Pseudoxanthomonas sp. DMVP2 isolated from hydrocarbon contaminated sediment of Amlakhadi canal, Gujarat, India”. Journal of Hazardous Materials, vol. 30, pp. 43-51, 2012.B. Ouided, and B. Abderrahmane, “Isolation and characterization of glyphosate-degrading bacteria from different soils of Algeria”. African Journal of Microbiology Research, vol. 7, No. 49, pp. 5587-559, 2013.

T.B. Moorman,”Pesticide degradation by soil microorganisms: environmental, ecological and management effects”. Advances in Soil Sciences, vol. 33, pp. 261-165, 1994

F. Jieyu, Y. Guoxia, Z. Haoyu, S. Guanying, G. Yucong, H. Taiping, and K. Tao, “Isolation, identification and characterization of a glyphosate-degrading bacterium, Bacillus cereus CB4, from soil”. Journal of General and Applied Microbiology, vol. 58, pp. 263-271, 2012.

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How to Cite
Aso, R. E., Hammuel, C., Daji, M., & Briska, J. (2021). Growth Optimization of Glyphosate-based Herbicides Utilizing Bacteria isolated from Lotic Water of Ogini Stream, Nigeria. European Journal of Biology and Biotechnology, 2(6), 1-5. https://doi.org/10.24018/ejbio.2021.2.6.290