Research Article

Antifungal Coating Based on Pyocyanin Nanoparticles (Np-Pyo)

Jonas Emanuel Guimarães da Silva
Universidade Federal da Paraíba, Brasil
Ian Porto Gurgel do Amaral
Universidade Federal da Paraíba, Brasil
Elisângela Afonso de Moura Kretzschmar
Universidade Federal da Paraíba, Brasil
Ulrich Vasconcelos
Universidade Federal da Paraíba, Brasil
* Corresponding author
DOI icon 10.24018/ejbio.2022.3.2.360

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Submitted 2022-03-14
Published 2022-04-21

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Table of Contents

Article Main Content

Pyocyanin is a pigment produced by 95% of Pseudomonas aeruginosa strains and exhibits antimicrobial properties that can be used for different purposes. In this work, PMMA-based nanoparticles that were encapsulated into 200 µg/mL of pyocyanin (Np-Pyo) were produced by the nanoprecipitation method. They were evaluated with respect to antifouling activity against Aspergillus sp. and Penicillium sp. With an encapsulation efficiency of 56%, the NpPyo remained stable for 90 days. Their characteristics were satisfactory for the following parameters: average size (616.90±38.30 nm; blank: 282.58±22.89 nm), polydispersion index (0.51±0.01; blank: 0.45±0.78), zeta potential (-5.13±0.41 mV; blank: -6.44±1.12 mV) and pH (6.18±0.03; blank: 6.42±0.01). The in vitro biofilm formation assay was performed on dolomite coupons measuring 1 cm2, on which the formulation was applied. There were tested conditions with and without immersion for 72h at 30 ºC. In the tests with the immersed coupons, there was fungal colonization; this was, however, lower than that observed in the control. A. niger decreased by 3 log units. No growth was observed on the coupons that were not immersed. The results were promising and demonstrated viability as a means of antifouling protection, particularly on dry surfaces.

Keywords: Antifouling, bioreceptivity, coating, pyocyanin

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