Potato (Solanum tuberosum L.) Plantlet Regeneration in Ammonium Nitrate Free Stock Solution-1 of Murashige & Skoog (MS, 1962) Plant Tissue Culture Medium



Abstract Views
809

Downloads
484

Citations

Share

##plugins.themes.bootstrap3.article.sidebar##

Submitted Aug 27, 2022
Published Oct 23, 2022
10.24018/ejbio.2022.3.5.413

Abstract viewed = 809 times

Table of Contents

##plugins.themes.bootstrap3.article.main##

Potato (Solanum tuberosum L.) plantlet regeneration potentiality was studied in ammonium nitrate (NH4NO3) free tissue culture medium. Four different treatments were designed from the compositions of the stock solution-01 of plant tissue culture medium. Those were -Treatment-1 (Stock solution -01 as recommended by Murashige & Skoog (1962), Treatment-2 (MS stock solution-01 without having NH4NO3), Treatment-3 (MS stock solution-01without NH4NO3 but other component had double concentration), Treatment-4 (Readymade MS powder, Duchefa Biocheme, The Netherland). Shoot length, shoot diameter, node number and leaf number per plantlet were highest in Treatment-4 at 14, 21 and 28 days after inoculation (DAI). Shoot regeneration parameters were statistically similar with the treatment-3 and the check treatment-1. But the check treatment-1 showed better result in root number and root length (cm) as compared to treatment-3 and treatment-4. The treatment-2 showed lowest result in each of the said parameter. The stock solution-01 which was formulated without ammonium nitrate and has double dose of other ingredient has the potentiality for potato plantlet regeneration, but it was not as suitable as Readymade MS powder (Duchafa, The Netherland).

Keywords: Ammonium nitrate, potato regeneration, stock solution-1, tissue culture medium

References

  1. BBS (2020). Statistical Yearbook of Bangladesh. Statistics Division, Agriculture Wing, Ministry of Planning, Govt. of the People’s Republic of Bangladesh, Dhaka.
     Google Scholar
  2. Badoni, A., & Chauhan, J. S. (2010). In vitro sterilization protocol for micropropagation of Solanum tuberosum cv. ‘KufriHimalini’. Academia Arena, 2(4), 24-27.
     Google Scholar
  3. Bashar, M. A., Hoque, M. E., Siddique, A. B., Seyfullah K, Sheuly K. N., Rahman, M. H., et al. (2021). Potentiality of urea as a substitute of ammonium nitrate in tissue culture media for potato plantlet regeneration. Plant Cell Biotechnology and Molecular Biology, 118-126.
     Google Scholar
  4. Bashar, M.A. (2016). Identification of a a new chemical as a substitute of Ammonium nitrate (NH4NO3) in MS media preparation for in vitro regeneration of potato (Solanumtuberosum L.) [Doctoral dissertation, Sher-e-Bangla Agricultural University].
     Google Scholar
  5. Cummings, J. H., Beatty, E. R., Kingman, S. M., Bingham, S. A., Englyst, H. N. (1996). Digestion and physiological properties of resistant starch in the human large bowel. Breeding and Nutrition Journal, 75, 733-747.
     Google Scholar
  6. Englyst, H. N., Kingman, S. M., Cummings, J. H., (1992). Classification and measurement of nutritionally important starch fractions. European Journal of Clinical Nutrition, 67, 46-49.
     Google Scholar
  7. Gamborg, O. L., Miller, A., Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50(1), 151-158.
     Google Scholar
  8. Hylla, S., Gostner, A., Dusel, G., Anger, H., Bartram, H. P., Christl, S. U., et al. Effects of resistant starch on colon in healthy volunteers: possible implications for cancer prevention. 1998.American Journal of Clinical Nutrition, 67, 136-142.
     Google Scholar
  9. Hena, H. (2016). Study on potato regeneration capability in ammonium nitrate free medium. [M.S. Thesis]. Sher-e-Bangla Agricultural University.
     Google Scholar
  10. Koleva, G. L., Sasa, M., Trajkova, F., Ilievski, M. (2012). Micropropagation of potato Solanum tuberosum L. Electronic Journal of Biology. 8(3), 45-49.
     Google Scholar
  11. Khadiga, G. A., Rasheid, S. M., Mutasim, M. K. (2015). Micro tuber induction of two potato (Solanum tuberosum L.) varieties namely, Almera and Diamant. International Research Journal of Biological Sciences, 4(3): 84-89.
     Google Scholar
  12. Linsmaier, E. M., Skoog, F. (1965). Organic Growth Factor Requirements of Tobacco Tissue Cultures. Physiologia Plantarum, 18, 100-127.
     Google Scholar
  13. Molla, M. M. H., Nasiruddin, K. M., Al-Amin, M., Khanam, D., Salam, M. A. (2011). Effect of Growth Regulators on Direct Regeneration of Potato. International Proceeding on Environment and Industrial Innovation, IPCBEE, 12.
     Google Scholar
  14. Motallebi-Azar, A., Kazemiani, S., Kiumavsie, F., Mohaddes, N. (2011). Shoot proliferation from node explants of potato (Solanum tuberosum CV. Agria). II. Effect of different concentration of NH4NO3, Hydrolyzed casein and BAP. Romanian Biotechnology Letters,16(3), 6181-6186.
     Google Scholar
  15. Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15, 473-497.
     Google Scholar
  16. Rahman, M. H., Haider, S. A., Hossain, M., Islam, R. (2011). Effect of potassium and ammonium nitrate media on in vitro growth response of potato (Solanum tuberosunL.). International Journal of Biosciences, 1(2), 54-57.
     Google Scholar
  17. Qureshi, M. A., Gul, Z., Ali, S., Khan, A. R. (2014). Comparative growth response of potato plantlets developed on liquid vs solidified (MS) medium using tissue culture technology. International Journal of Recent Scientific Research, 5(4), 736-739.
     Google Scholar
  18. Wakil, M. A. (2020). Comparative performance of ammonium nitrate and other nitrogenous salts for in vitro regeneration of potato (Solanum tuberosum L.) [M.S. Thesis]. Sher-e-Bangla Agricultural University.
     Google Scholar