Effects of Ilex paraguariensis A. St.-Hil. (Yerba Mate) and Chlorogenic Acid on Creatine Phosphokinase, Lactate and Irisin Levels in Mice after Detraining


  •   Evandro Toledo Gerhardt Stutz

  •   Roberto Laureano-Melo

  •   Vladimir Lopes de Souza

  •   Geraldo Ceni Coelho

  •   Claudia Cardoso Netto

  •   Carlos Alberto Bastos de Maria


This is the first report that deals with the effect of yerba mate (YM) and chlorogenic acid (CGA) consumption on blood total creatine phosphokinase (CPK), lactate and irisin levels after a detraining period. Healthy mice (n = 50) were randomly separated into the following experimental groups: SED = sedentary control; TRAIN = mice submitted to training (swimming) for 8 weeks; DETRAIN = mice submitted to training for four weeks and after submitted to detraining for four weeks; DETRAIN CGA = mice submitted to training for four weeks and after submitted to both detraining and standard CGA consumption for four weeks; DETRAIN YM = mice submitted to training for four weeks and after submitted to both detraining and consumption of roasted YM infusion for four weeks. No significant (p > 0.05) difference was found in relation to the lactate value. The DETRAIN CGA group had a lower CPK data when compared with the other groups. The DETRAIN YM group showed a lactate value slightly lower than that from DETRAIN CGA one. Blood irisin value had no statistical (p > 0.05) difference between studied groups. Irisin value in sedentary group had a value non-statistically (p > 0.05) significant when compared with the other groups after a detraining period. Therefore, CGA could decrease the protein catabolism via CPK inhibition during detraining. YM may maintain a part of the lipid mobilization during detraining, leading to the sparing of a fraction of the lactate.

Keywords: Ylex paraguariensis, mice, detraining, CPK, lactate, irisin

Keywords: CPK, detraining, lactate, irisin, mice, Ylex paraguariensis


Alkhatib A. (2014). Yerba Maté (Ilex paraguariensis) ingestion augments fat oxidation and energy expenditure during exercise at various submaximal intensities. Nutrition and Metabolism, 11, 42.

Cazal, M.D.M. (2019). Efeito da ingestão de chá mate (Ilex paraguariensis) antes do exercício aeróbico sobre indicadores metabólicos de dano muscular e estresse oxidativo [Doctoral dissertation, Federal University of Viçosa]. Portuguese.

de Souza, V.L., Stutz, E.T.G., Pehrson, M.E., Coelho, G.C., Netto, C.C., de Maria, C.A.B. (2021). Ilex paraguariensis A. St.-Hil. (Yerba Mate) differently regulates the lipid mobilization and irisin and lactate levels in sedentary and chronic swimming mice. European Journal of Medical Health Sciences, 3(6), 58-62.

Fernandes, E.S., Machado, M.O., Becker, A.M., Andrade, F., Maraschin, M., da Silva, E.L. (2012). Yerba mate (Ilex paraguariensis) enhances the gene modulation and activity of paraoxonase -2: In vitro and in vivo studies. Nutrition, 28, 1157-1164.

Hermmele, H., Burger, H.J., Below, P., Schubert, G., Rippel, R., Schindler, P.W., et al. (1997). Chlorogenic acid and synthetic chlorogenic acid derivatives: novel inhibitors of hepatic glucose-6-phosphate translocase. Journal of Medicinal Chemistry, 40, 137-145.

Junior, E.L.C., & Morand, C. (2016). Interest of mate (Ilex paraguariensis A. St.- Hil.) as a new natural functional food to preserve human cardiovascular Healthy – a review. Journal of Functional Foods, 21, 440-444.

Moura, B.M., Panza, V.P., Brunetta, H.S., Tamborindeguy, A.C., de Oliveira M.V., Sakugawaet R.F. et al. (2020). Effect of mate tea consumption on rapid force production after eccentric exercise: a randomized, controlled, crossover study. Sport Sciences for Health, 6, 1-11.

Ong, K.W., Hsu, A., Tan, B.K.H. (2013). Anti-diabetic and anti-lipidemic effects of chlorogenic acid are mediated by AMPK activation. Biochemical Pharmacology, 85, 1341-1351.

Riachi, L.G., & de Maria, C.A.B. (2017). Yerba mate: An overview of physiological effects in humans. Journal of Functional Foods, 38, 308-320.

Riachi, L.G., Marcellini, P.S., de Maria, C.A.B., Coelho, G.C. (2019). Yerba mate consumption effect on the total concentration of creatine phosphokinase in healthy volunteers from of age of 50. European Journal of Medical Health Sciences, 1, 1-3.

Riachi, L.G., Simas D.R.L., Coelho, G.C., Marcellini, P.S., da Silva, A.J.R., de Maria, C.A.B. (2018). Effect of light intensity and processing conditions on bioactive compounds in mate extracted from yerba mate (Ilex paraguariensis A. St.-Hil.). Food Chemistry, 266, 317-322.

Ribeiro, M.C., Santos, A., Riachi, L.G., Rodrigues, A.C.B., Coelho, G.C., Marcellini, P.S., et al. (2017). The effects of roasted yerba mate (Ilex paraguariensis A. St. Hil.) consumption on glycemia and total serum creatine phosphokinase in patients with traumatic brain injury. Journal of Functional Foods, 28, 240-245.

Xu, M., Yang, L., Liao, M., Chu, L., Li, X. (2019). Collaborative effects of chlorogenic acid and caffeine in lipid metabolism via of AMPKα-LXRα/SREBP-1c pathway in high-fat diet-induced obese mice. Food Function,10, 7489-7497.


How to Cite
Toledo Gerhardt Stutz, E., Laureano-Melo, R., de Souza, V. L., Coelho, G. C., Netto, C. C., & de Maria, C. A. B. (2023). Effects of Ilex paraguariensis A. St.-Hil. (Yerba Mate) and Chlorogenic Acid on Creatine Phosphokinase, Lactate and Irisin Levels in Mice after Detraining. European Journal of Biology and Biotechnology, 4(1), 12–15. https://doi.org/10.24018/ejbio.2023.4.1.419