In vitro Exopolysaccharide Production of Some Soil Bacteria Isolated from Soil Affected by Salt



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Submitted Jun 30, 2020
Published Jul 16, 2020
10.24018/ejbio.2020.1.4.56

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In this study, production of exopolysaccharide (EPS) from soil bacteria isolated from the salinity soil and the effects of agitation, some carbon sources such as glucose, fructose, xylose, rhamnose and NaCl levels the on production of polysaccharide playing a role in soil aggregation were studied. Maximum EPS production for Acinetobacter sp., Pseudomonas fluorescens and Sphingomonas paucimobilis was obtained 72 h of incubation and maximum EPS production for Bacillus simplex was obtained at 96 h. Maximum EPS production was obtained at 150 rpm for all bacteria studied. Glucose gave the best result for all of bacteria. Bacillus simplex produced higher EPS and viscosity than the other bacteria. NaCl levels affected both the growth and exopolysaccharide production of the bacteria. By promoting aggregate formation, EPS produced bacteria such as S.paucimobilis, B.simplex, Acinetobacter sp. and P.fluorescens may have created favorable growth environments.

Keywords: Soil bacteria, carbon source, NaCl, agitation, exopolysaccharide, salinity soil

References

  1. S. Cazrnes, P. D. Hallett, A.G. Bengough, and I. M. Yang. “Root and microbial derived mucilages affect soil structure and water transport”. Eur J Soil Sci. vol. 51, pp. 435-443, 2000.
     Google Scholar
  2. S. K. Upadhyay, J. S. Singh, and D. P. Singh. “Exopolysaccharide producing plant growth promoting rhizobacteria under salinity condition”, Pedosphere. vol. 21, pp. 214-222, 2011.
     Google Scholar
  3. T.T More, J.S.S. Yadav, S. Yan, R. D. Tyagi, and R.Y. Surampalli. “Extracellular polymeric substances of bacteria and their potential environmental applications” Journal of Environmental Management. vol. 144, pp.1-25, 2014.
     Google Scholar
  4. N. Amellal, G. Burtin, F. Bartoni, and T. Heulin. 1998. “Colonization of wheat roots by EPS producing Pantoea agglomerans and its effect on rhizosphere soil aggregation,” Appl Environ Microbiol. vol.64, pp. 3740-3747, 1998.
     Google Scholar
  5. D.M. Mager, and A.D. Thomas. “Extracellular polysaccharides from cyanobacterial soil crusts: A review of their role in dryland soil processes,” J Arid Environ. vol. 75, pp. 91-97, 2011.
     Google Scholar
  6. M. Ashraf, O. Berge, F. Azam, and T. Heulin. “Bacterial exopolysaccharides and productivity of salt affected soils. I. Diversity of exopolysaccharides producing bacteria isolated from the rhizosphere of wheat (Triticum aestivum L.) grown in normal and saline Pakistani soils,” Pak J Biol Sci, vol. 2, pp. 201-206, 1998.
     Google Scholar
  7. S. Tewari, and N.K. Arora. “Multifunctional exopolysaccharides from Pseudomonas aeruginosa PF23 involved in plant growth stimulation, biocontrol and stress amelioration in sunflower under saline conditions”, Curr. Microbiol. vol. 69, pp. 484-494, 2014.
     Google Scholar
  8. Ç. Küçük, and M. Kıvanç. “Extracellular polysaccharide production by Rhizobium ciceri from Turkey”, Annals of Microbiol. vol. 59, pp. 141-144, 2009.
     Google Scholar
  9. C. Vanhaverbeke, A. Heyraud, and K. Mazeau. “Conformational analysis of the exopolysaccharide from Burkholderia caribensis strain. MWAP71: impact on the interaction with soils”, Biopolymers, vol. 69, pp. 480-497, 2003.
     Google Scholar
  10. Y. S. Kang, and W. Park. “Protection against diesel oil toxicity by sodium chloride-induced exopolysaccharides in Acinetobacter sp. strain DRI”, J Biosci Bioengin. vol. 109, pp. 118-123, 2010.
     Google Scholar
  11. S. A. Razack, V. Velayutham, and V. Thangavelu. “Medium optimization for the production of exopolysaccharide by Bacillus subtilis using synthetic sources and agro wastes”, Turk J Biol. vol. 37, pp. 280-288, 2013.
     Google Scholar
  12. J. L. Geddie, and I.W. Sutherland. “Uptake of metals by bacterial polysaccharides”, J Appl Bacteriol. Vol. 74, pp. 467-472, 1993.
     Google Scholar
  13. E. Jofre, S. Fischer, V. Rivarola, H.G. Balegno, and G. Mori. “Saline stress affects the attrachment of Azospirillium brasilense Cd to maize and wheat root”, Can J Microbiol., vol.44, pp.416-422, 1998.
     Google Scholar
  14. J. H. Suh, J. W. Yun, and D. S. Kim. “Effect of extracellular polymeric substances (EPS) on Pb2+ accumulation by Aureobasidium pullulans”, Bioprocess Biosyst Eng. Vol. 21, pp. 1-4, 2013.
     Google Scholar
  15. D. Lobas, S. Schumpe, and W. D. Deckwer. “The production of gellan exopolysaccharide with Spingomonas paucimobilis E2 (DSM-6314). Appl Microbiol Biotechnol. vol. 37, pp. 411-415, 1992.
     Google Scholar
  16. C. Dupraz, and K. P. T. Vissher. “Microbial lithification in marine stromatolites and hypersaline mats”, Trends Microbiol. vol.13, pp. 429-438, 2005.
     Google Scholar
  17. P. Joshi, and A. Juwarkar. “In vivo studies to elucidate the role of extracellular polymeric substances from Azotobacter in immobilization of heavy metals”, Environ Sci Technol. vol. 43, pp. 5884-5889, 2009.
     Google Scholar
  18. M. Ashraf, S. Hasnain, O. Berge, and T. Mahmood. “Inoculating wheat seedlings with exopolysaccharide producing bacteria restricts sodium uptake and stimulates plant growth under salt stress”, Biol Fertil Soils. vol. 40, pp.157-162, 2004.
     Google Scholar
  19. C. Chenu, and J. Guerif. “Mechanical strength of clay minerals as influenced by an absorbed polysaccharide”, Soil Sci Soc Am J. vol. 55, pp. 1076-1080, 1991.
     Google Scholar
  20. M. A. Çullu, A. Almaca, A.R. Öztürkmen, N. Ağca, F. İnce, R. Derici, and A. Seyrek. “Harran ovası topraklarında tuzluluğun yayılma olasılığının belirlenmesi”, GAP BKİ Tarımsal Projeler Kesin Sonuç Raporu, Proje No: 4-1, pp.1-72, 2000. (in Turkish)
     Google Scholar
  21. D. L. Rowell. “Soil Science: Methods and Applications” Longman, Harlow, Essex; New York: Longman Scientific & Technical; Wiley, ISBN 0-592-087848, 1994.
     Google Scholar
  22. A. E. Martin, and R. Reeve. “A rapid manometeic method for determining soil carbonate”, Soil Science, vol. 79, pp. 187-198, 1955.
     Google Scholar
  23. A. Walkey. “A critical examination of a rapid method for determining organic carbon in soils effect of variations in digestion conditions and of inorganic soil constituents”, Soil Science. vol. 63, pp. 251-263, 1964.
     Google Scholar
  24. T. Heulin, A. Guckert, and J. Balandreau. “Stimulation of root exudation of rice seedlings by Azospirillum strains:carbon budget under gnotobiotic conditions”, Biol Fertil Soils. vol. 4, pp. 9-11, 1987.
     Google Scholar
  25. M. Dubois, K. A. Gilles, J. K. Hamilton, P. A. Rebers and F. Smith. “Colorimetric method for determination of sugars and related substanes”, Anal Chem. vol. 28, pp. 350-356, 1956.
     Google Scholar
  26. U.U. Nwodo, E. Green, and A.L. Okoh. “Bacterial exopolysaccharides: functionality and prospects”, Int. J. Mol. Sci. vol. 13, pp. 14002-14015, 2012.
     Google Scholar
  27. R. M. Banik, and A. Santhiagu. “Improvement in production and quality of gellan gum by Sphingomonas paucimobilis under high dissolved oxygen tension levels”, Biotech Lett. vol. 28, pp.1347-1350, 2006.
     Google Scholar
  28. L. Navarini, M. Stredansky, M. Matulova, and C. Bertocchi. “Production and characterization of an exopolysaccharide from Rhizobium hedysari HCNT 1”, Biotechnology Lett. vol. 19, pp. 1231-1234, 1997.
     Google Scholar
  29. M. W. Breedveld, L.P.T. Zevenhevizen, H.C.J. Conter-Cremes, and A.J.B. Zehnder. “Influence of growth conditions on production of capsular and extracellular polysaccharides by Rhizobium leguminosarum”, Ant Leeuw Microbiol. vol. 64, pp.1-8, 1993.
     Google Scholar
  30. M. Aguilera, M.T. Quesada, V.G.A. Guila, J.A. Morillo, A.R. Rivadeneyra, T. Cormenzana, and M.M. Sanchez. “Characterization of Paenibacillus jamilae strains that produce exopolysaccharide during growth on and detoxification of olive mill wastewaters”, Bioresour Technol. vol. 99, pp. 5640-5644, 2008.
     Google Scholar
  31. I. P. Sen, A.K. Mandal, R. Chakraborty, B. Behera, K.K. Yadav, T.K. Maiti, and S.S. Islam. “Structural and immonological studies of an exopolysaccharide from Acinetobacter junii BB1A”, Carbohydrate Polymers. vol. 101, pp. 188-195, 2014.
     Google Scholar
  32. H. Dalton, and J.R. Postgate. “Effect of oxygen on growth of Azotobacter chroococcum in batch and continous culture”, J Gen Microbiol. vol. 54, pp. 463-471, 1968.
     Google Scholar
  33. A. Elkines, E. Rosenberg, and E.Z Ron. “Production and secretion of polysaccharide biodispersan of Acinetobacter calcaaceticus A2 in protein secretion mutants”, Appl Environ Microbiol., vol. 60, pp. 4642-4645, 1994.
     Google Scholar
  34. S. T. Pawar, A.A. Bhosale, T.B. Gawade, and T.R. Nale. “Isolation, screening and optimization of exopolysaccharide producing bacterium from saline soil”, J Microbiol Res. vol. 3, pp. 24-31, 2013.
     Google Scholar
  35. H. H. Zahran. “Diversity, adaptation and activity of the bacterial flora in saline environments”, Biology and Fertility Soils. vol. 25, pp. 211-223, 1997.
     Google Scholar
  36. D. Or, S. Phutane, and A. Dechesne A. “Extracellular polymeric substances affecting pore-scale hydrologic conditions for bacterial activity in unsaturated soils”, Vadose Zone Journal. vol. 6, pp. 298–305, 2007.
     Google Scholar
  37. M. A. Hanjra, and M.E. Qureshi. “Global water crisis and future food security in an aera of climate change”, Food Policy. vol. 35, pp. 365-377, 2010.
     Google Scholar