Efficacy of Benzylkonium and Clorhexidine against Clinical Isolates of Pseudomonas Sp. from Dhaka, Bangladesh



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Submitted Jul 17, 2022
Published Aug 19, 2022
10.24018/ejbio.2022.3.4.389

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The opportunistic pathogen Pseudomonas aeruginosa has been recognized as a major cause of nosocomial infection due to resistance to multiple classes of antibiotics. This study reports presence of carbapenem resistance genes in clinical Pseudomonas isolates from Dhaka, Bangladesh, as well as their resistance to some common disinfectants (benzylkonium chloride and chlorhexidine gluconate). 

Twenty three samples were collected from two hospitals and one diagnostic center from different locations in Dhaka city. Among these, 16 isolates were identified as Pseudomonas sp. from growth on Cetrimide Agar, standard biochemical tests (catalase, oxidase and triple sugar iron, urea, Sulphur Indole Motility medium, Lysine Iron Agar, Methyl Red-Voges Proskaeur medium, nitrate, citrate) and IasL/R-gene targeted PCR. Molecular characterization revealed three of the isolates contained 2.3kb to 2.5 kb plasmids. The genome of 6 isolates contained blaGIM gene encoding metallo-beta-lactamase variant (German), but none of isolates were resistant against imipenem group of antibiotics (meropenem, gentamicin, cefixime, imipenem). Therefore the isolates are genetically potent to resist antibiotics belonging to imipenem group, but are still sensitive to the antibiotics in culture. 

3% Benzylkonium chloride and 0.5% chlorhexidine gluconate has slightly synergistic effect in inhibiting Pseudomonas sp. in representative isolates as their Fractional Inhibitory Concentration Index value ranges between 0.22 and 0.5. Taken together, Pseudomonas sp. isolates harboring potential virulent genes and imipenem-resistance genes could be inhibited more effectively in vitro if more than one suitable disinfectants are applied combinedly.

Keywords: blaIMP, disinfectant, metallo-β-lactamase, pseudomonas

References

  1. Aghamollaei H, Moghaddam MM, Kooshki H, Heiat M, Mirnezad R, Bazri N. (2015). Detection of Pseudomonas aeruginosa by triples polymerase chain reaction using IasI/R and GyrB genes. Journal of Infection and Public Health, 8(4):314-322.
     Google Scholar
  2. Balasubramanian, D., Kumari, H., Jaric, M., Fernandez, M., Turner, K. H., et al. (2014). Deep sequencing analyses expands the. Nucleic Acids Research, 42(2), 979-998.
     Google Scholar
  3. Golia, S., Suhani, Manasa, S., Jyoti. (2016). Isolation of Pseudomonas aeruginosa from various Clinical Isolates and it. International Journal of Current Microbiology and Applied Sciences, 5(3), 247-253.
     Google Scholar
  4. Hu, P., Chen, J., Chen, Y., Zhou, T., Xu X., Pei, X. (2017). Molecular epidemiology, resistance, and virulence properties of. Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases, 55, 288-296.
     Google Scholar
  5. Iglewski, B. H. Pseudomonas. (1996). Medical Microbiology. 4th ed., The University of Texas Medical Branch at Galveston.
     Google Scholar
  6. Lila, G., Mulliqi, G., Raka, L., Kurti, A., Bajramielvir R., Azizi, A. (2018). Molecular epidemiology of Pseudomonas. Infection and Drug Resistance, 11, 2039–2046.
     Google Scholar
  7. Mukherjee, K., Tribedi, P., Mukhopadhyay, B., Sil, A. K. (2013). Antibacterial Activity of long-chain fatty alcohol against mycobacteria. FEMS Microbiol Lett, 338(2), 177–183.
     Google Scholar
  8. Nagaveni, S., Rajeshwari, H., Oli A. K., Patil, S. A., Chandrakanth, R. K. (2011). Widespread Emergence of Multidrug Resistant Pseudomonas. Indian J Microbiol, 51, 2–7.
     Google Scholar
  9. National Center for Emerging and Zoonotic Infectious Diseases (NCEZID). (2018 March). Organisms, Diseases and. Centers for Disease Control and Prevention. https://www.cdc.gov/hai/organisms/pseudomonas.html.
     Google Scholar
  10. Patel, J. B., Weinstein, M. P., Eliopoulos, G. M., Patel, R., Richter, S. S. (2017).Performance Standards for Antimicrobial Susceptibility Testing. 27th ed. Clinical and Laboratory Standards Institute M100.
     Google Scholar
  11. Sambrook, J., Russel D. W. (2006). Purification of nucleic acids by extraction with phenol: chloroform: isoamyl alcohol CSH Protoc, 1, pdb.prot4455.
     Google Scholar
  12. Performance standards to antimicrobial susceptibility testing (27th edition) (M-100) (2017) Clinical Laboratory Standard Institute, USA
     Google Scholar
  13. Wickham Laboratories. https://wickhamlabs.co.uk/technical-resource-centre/fact-sheet-pseudomonas-aeruginosa/.
     Google Scholar
  14. Yoon, E., Jeong, S. H. (2021). Mobile carbapenamase genes in Pseudomonas aurginosa. Frontiers in Microbiol, 12, 614058.
     Google Scholar


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